Selected Cell
Cell:
Value:
D33_Agrob-80
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| Plasmid | Target gene | Terminator | Promoter | Signal | Tag | Comment | Strain | ||
| Icon 3'-module pICH008 2D6 | H_CYP2D6_M | nos | ACT2 | HIS | Icon viral system pICH008: 3'-module CYP2D6 | A. t. GV3101 | |||
| Icon 3'-module pICH008 3A4 | H_CYP3A4_M | nos | ACT2 | HIS | Icon viral system pICH008: 3'-module CYP3A4 | A. t. GV3101 | |||
| Icon 3'-module pICH008 GFP | H_sGFP_M | nos | ACT2 | HIS | Icon viral system pICH008: 3'-module GFP | A. t. GV3101 | |||
| Icon 3'-module pICH008 LicBM3 | H_LicBM3_M | nos | ACT2 | HIS | Icon viral system pICH008: 3'-module LicBM3 | A. t. GV3101 | |||
| Icon 3'-module pICH008 sGFP::LicBM3 | H_sGFP::LicBM3_M | nos | ACT2 | HIS | Icon viral system pICH008: 3'-module sGFP::LicBM3 | A. t. GV3101 | |||
| Icon 3'-module pICH008_mCherry | H_mCherry_M | nos | ACT2 | HIS | Icon viral system pICH008: 3'-module mCherry | A. t. GV3101 | |||
| Icon 3'-module pICH011 1A2 | G_1A2_M | nos | ACT2 | HIS | Icon viral system pICH011: 3'-module 1A2 | A. t. GV3101 | |||
| Icon 3'-module pICH011 2D6 | G_2D6_M | nos | ACT2 | HIS | Icon viral system pICH011: 3'-module 2D6 | A. t. GV3101 | |||
| Icon 3'-module pICH011 3A4 | G_3A4_M | nos | ACT2 | HIS | Icon viral system pICH011: 3'-module 3A4 | A. t. GV3101 | |||
| Icon 3'-module pICH011 GFP | G_sGFP_M | nos | ACT2 | HIS | Icon viral system pICH011: 3'-module GFP | A. t. GV3101 | |||
| Icon 3'-module pICH011_2D6::GFP | G_2D6::GFP_M | nos | ACT2 | - | HIS | Icon viral system pICH011: 3'-module 2D6::GFP | A. t. GV3101 | ||
| Icon 3'-module pICH011_CTP_GFP::LicBM3 | H_GFP::LicBM3_M | nos | ACT2 | CTP | HIS | Icon viral system pICH011: 3'-module for chloroplast targeting of GFP::LicBM3 | A. t. GV3101 | ||
| Icon 5'-module pICH15879 no targeting (intron) | cr-TMV Movement Protein (MP) | nos | ACT2 | - | Icon viral system pICH15879: 5'-module no targeting (intron) | A. t. GV3101 | |||
| Icon 5'-module pICH20030 chl targ (intron) | cr-TMV Movement Protein (MP) | nos | ACT2 | CTP | - | Icon viral system pICH20030: 5'-module chloroplast targeting (intron) | A. t. GV3101 | ||
| Icon recombinase pICH10881 | PhiC31 integrase | nos | ACT2 | - | Icon viral system pICH10881: PhiC31 integrase | A. t. GV3101 | |||
| pDGB2_alpha1_YA28_P35S_CTP_GFP_LicBM3_HIS_Tnos | H_GFP::LicBM3_M_HIS_N | nos | 35S | CTP | HIS | pDGB2 TU GFP::LicBM3 HIS tag (reporter), plast. loc. | A. t. EHA105 | ||
| pDGB2_alpha1_YA29_P35S_G_gammaTIP_M_mCherry_N_Tnos | G_gammaTIP_M_mCherry_N | nos | 35S | - | - | Vacuolar gamma-TIP C-fused with mCherry | A. t. EHA105 | ||
| pDGB2_alpha1_YA30_P35S_G_deltaTIP_M_mCherry_N_Tnos | G_deltaTIP_M_mCherry_N | nos | 35S | - | - | Vacuolar delta-TIP C-fused with mCherry | A. t. EHA105 | ||
| pDGB2_alpha1_YA32_P35S_G_deltaTIP_M_LssmOranN_Tnos | G_deltaTIP_M_LssmOrange_N | nos | 35S | - | - | Vacuolar delta-TIP C-fused with LssmOrange (shifted stop) | A. t. EHA105 | ||
| pDGB2_alpha1_YA35_P35S_Y25hNPRopt_TNos | G_hNPRopt_N | nos | 35S | - | - | Human NADPH P450 reductase (hNPR) optimized for N.b expression | A. t. EHA105 | ||
| pDGB2_alpha1_YA36_P35S_Y24AtR1_TNos | G_AtR1var_N | nos | 35S | - | - | A.thaliana NADPH-cytochrome P450 reductase (X66016), var. S161Y; CQDW477-481SPRL | A. t. EHA105 | ||
| pDGB2_alpha1_YA37_P35S_CTP_Y26hNPRopt_TNos | H_hNPRopt_N | nos | 35S | CTP | - | Human NADPH P450 reductase (hNPR) optimized for N.b expression | A. t. EHA105 | ||
| pDGB2_alpha1_YA38_P35S_CTP_Y24AtR1_TNos | H_AtR1var_N | nos | 35S | CTP | - | A.thaliana NADPH-cytochrome P450 reductase (X66016), var. S161Y; CQDW477-481SPRL | A. t. EHA105 | ||
| pDGB2_alpha1_YA45_P35S_Y23AtR2_TNos.gb | G_AtR2_N | nos | 35S | - | - | A.thaliana NADPH-cytochrome P450 reductase (AF325101.1) | A. t. EHA105 | ||
| pDGB2_alpha1_YA46_P35S_Y35CrCPR1_TNos | G_CrCPR1_N | nos | 35S | - | - | C. rhoseus CrCPR1 reductase KJ701028.1 | A. t. EHA105 | ||
| pDGB2_alpha1_YA47_P35S_Y35CrCPR2_TNos | G_CrCPR2_N | nos | 35S | - | - | C. rhoseus CrCPR2 reductase X69791.1 | A. t. EHA105 | ||
| pDGB2_alpha1_YA48_P35S_Y40NbCPR1_TNos | G_NbCPR1_N | nos | 35S | - | - | N. benthamiana NbCPR1 reductase Nbv6.1trP59491 | A. t. EHA105 | ||
| pDGB2_alpha1_YA49a_P35S_Y41NbCPR2intron_TNos | G_NbCPR2_N | nos | 35S | - | - | N. benthamiana NbCPR2 reductase Nbv6.1trP32999 | A. t. EHA105 | ||
| pDGB2_alpha1_YA50_P35S_Y14_G_2D6_M_mCherry_N_Tnos | G_2D6_M_mCherry_N | nos | 35S | - | - | pDGB2 TU 2D6 fused with mCherry | A. t. EHA105 | ||
| pDGB2_alpha1_YA51_P35S_Y14_G_2D6_M_LssmOrange_N_Tnos | G_2D6_M_LssmOrange_N | nos | 35S | - | - | pDGB2 TU 2D6 fused with LssmOrange | A. t. EHA105 | ||
| pDGB2_alpha1_YA52_P35S_Y14_G_2D6_M_LicBM3_N_Tnos | G_2D6_M_LicBM3_N | nos | 35S | - | - | pDGB2 TU 2D6 fused with LicBM3 | A. t. EHA105 | ||
| pDGB2_alpha1_YA53_P35S_Y42DmCPR1_TNos | G_DmCPR1_N | nos | 35S | - | - | D. melanogaster reductase X93090.1 N.benth-expression optimized in alpha1 unit | A. t. EHA105 | ||
| pDGB2_alpha1_YA54_P35S_CTP_Y15_H_2D6_M_LssmOrange_N_Tnos | H_2D6_M_LssmOrange_N | nos | 35S | CTP | - | pDGB2 TU 2D6 fused with LssmOrange, chloroplast targeting | A. t. EHA105 | ||
| pDGB2_alpha1_YA54_P35S_CTP_Y15_H_2D6_M_mCherry_N_Tnos | H_2D6_M_mCherry_N | nos | 35S | CTP | - | pDGB2 TU 2D6 fused with mCherry, chloroplast targeting | A. t. EHA105 | ||
| pDGB2_alpha1_YA56_P35S_Y43Nbcyb5_TNos | G_Nbcyb5_N | nos | 35S | - | - | Nicotiana benthamiana synthetic Nbv6.1trP16907_cypb5 | A. t. EHA105 | ||
| pDGB2_alpha1_YA59_P35S_Y44_G_DeltaTIP_H_Y38_H_2D6_GFP_N_Tnos | G_DeltaTIP_H_2D6_GFP_N | nos | 35S | DeltaTIP | - | human P450 2D6 fused with GFP and At DeltaTIP at N-terminal | A. t. EHA105 | ||
| pDGB2_alpha1_YA62_P35S_G_AtWAK2_ERsig_H_mCherry_M_HDEL_Tnos | G_AtWAK2_ERsig_H_mCherry_M_HDEL | nos | 35S | ER_HDEL | - | mCherry fused with At ER signal sequence (N) and HDEL (C) | A. t. EHA105 | ||
| pDGB2_alpha1_YA63_P35S_G_AtWAK2_ERsig_H_sGFP_M_HDEL_Tnos | G_AtWAK2_ERsig_H_sGFP_M_HDEL | nos | 35S | ER_HDEL | - | sGFP fused with At ER signal sequence (N) and HDEL (C) | A. t. EHA105 | ||
| pDGB2_alpha1_YA64_P35S_G_Nbcyb5Red_N_Tnos | G_Nbcyb5Reductase_N | nos | 35S | - | - | N. benthamiana cyb5 reductase PX442347 | A. t. EHA105 | ||
| pDGB2_alpha2_YA65_P35S_G_AtWRI1ddhis_Tnos | G_WRI1ddhis_N | nos | 35S | - | - | A.t WRINKLED1 (WRI1) is a key transcription factor governing plant oil biosynthesis | A. t. EHA105 | ||
| pDGB2_Omega1_YO59_LxiLPPS_1A2 | LPPS; 1A2 | nos;nos | 35S;35S | - | - | For transient expression N.benthamiana lavandula x intermedia LPPS and 1A2 in omega 1 | A. t. EHA105 | ||
| pDGB2_Omega1_YO63_NsCPPS_2A6 | CPPs;2A6 | nos;nos | 35S;35S | - | - | For transient expression Nicotiana silvestris CPPS and 2A6 in omega 1 | A. t. EHA105 | ||
| pDGB2_Omega1R_YO04_YA04_p19_YA01_6G1 | LB_p19; CYP6G1_RB | nos;nos | 35S; 35S | -;CTP | For transient expression (T-P;T-P orientation) | A. t. EHA105 | |||
| pDGB2_Omega1R_YO05_YA04_p19_YA02_CYP2D6 | LB_p19; CYP2D6_RB | nos;nos | 35S; 35S | CTP;- | - | For transient expression CTP (T-P;T-P orientation) | A. t. EHA105 | ||
| pDGB2_Omega1R_YO10_YA04_p19_YA07_6G1 | LB_p19; CYP6G1_RB | nos;nos | 35S; 35S | -;native | For transient expression (T-P;T-P orientation) | A. t. EHA105 | |||
| pDGB2_Omega1R_YO11_YA04_p19_YA08_2D6 | LB_p19; 2D6_RB | nos;nos | 35S; 35S | -;native | - | For transient expression cyt. loc | A. t. EHA105 | ||
| pDGB2_Omega1R_YO12_YA04_p19_YA09_GFP_Lic | LB_p19; sGFP::LicBM3_RB | nos;nos | 35S; 35S | -;- | - | For transient expression cyt. loc | A. t. EHA105 | ||
| pDGB2_Omega1R_YO13_YA04_p19_YA06_CTP_3A4 | LB_p19; 3A4_RB | nos;nos | 35S; 35S | -;CTP | - | For transient expression cyt. loc | A. t. EHA105 | ||
| pDGB2_Omega1R_YO14_YA04_p19_YA10_3A4 | LB_p19; 3A4_RB | nos;nos | 35S; 35S | -;native | - | For transient expression cyt. loc | A. t. EHA105 | ||
| pDGB2_Omega1R_YO17_YA11_nptII_YA07_6G1 | LB_nptII; 6G1_RB | 35S;nos | nos; 35S | -;- | - | For transformation | A. t. GV3101 | ||
| pDGB2_Omega1R_YO17_YA11_nptII_YA07_6G1 | LB_nptII; 6G1_RB | 35S;nos | nos; 35S | -;- | - | For transformation Italy's strain | A. rh. A4 It | ||
| pDGB2_Omega1R_YO18_YA11_nptII_YA08_2D6 | LB_nptII; 2D6_RB | 35S;nos | nos; 35S | -;- | - | For transformation | A. t. GV3101 | ||
| pDGB2_Omega1R_YO18_YA11_nptII_YA08_2D6 | LB_nptII; 2D6_RB | 35S;nos | nos; 35S | -;- | - | For transformation Italy's strain | A. rh. A4 It | ||
| pDGB2_Omega1R_YO19_YA11_nptII_YA09_GFP_Lic | LB_nptII; GFP::LicBM3_RB | 35S;nos | nos; 35S | -;- | - | For transformation | A. t. GV3101 | ||
| pDGB2_Omega1R_YO19_YA11_nptII_YA09_GFP_Lic | LB_nptII; GFP::LicBM3_RB | 35S;nos | nos; 35S | -;- | - | For transformation Italy's strain | A. rh. A4 It | ||
| pDGB2_Omega1R_YO20_YA11_nptII_YA10_3A4 | LB_nptII; 3A4_RB | 35S;nos | nos; 35S | -;- | - | For transformation Italy's strain | A. rh. A4 It | ||
| pDGB2_Omega1R_YO22_YA04_p19_YA22_2D6_HIS | p19; H_2D6_M_HIS_N | nos;nos | 35S;35S | CTP | HIS | For transient expression plast. loc. HIS tag | A. t. EHA105 | ||
| pDGB2_Omega1R_YO29_YA04_p19_YA08_2D6_HIS | LB_p19; 2D6_M_HIS_N_RB | nos;nos | 35S; 35S | -;native | HIS | For transient expression cyt. loc | A. t. EHA105 | ||
| pDGB2_Omega1R_YO30_YA04_p19_YA35_hNPRopt | p19; G_hNPRopt_N | nos;nos | 35S;35S | - | - | For transient expression Human NADPH P450 reductase (hNPR) optimized | A. t. EHA105 | ||
| pDGB2_Omega1R_YO31_YA04_p19_YA36_ATR1 | p19; G_ATR1_N | nos;nos | 35S;35S | - | - | For transient expression A. thaliana AtR1 reductase with ins/del | A. t. EHA105 | ||
| pDGB2_Omega1R_YO32_YA04_p19_YA37_CTP_hNPRopt | p19; H_hNPRopt_N | nos;nos | 35S;35S | CTP | - | For transient expression Human NADPH P450 reductase (hNPR) optimized | A. t. EHA105 | ||
| pDGB2_Omega1R_YO33_YA04_p19_YA38_CTP_ATR1var | p19; G_ATR1_N | nos;nos | 35S;35S | CTP | - | For transient expression A. thaliana AtR1 reductase with ins/del with CTP | A. t. EHA105 | ||
| pDGB2_Omega1R_YO34_YA04_p19_YA39_2D6insvac | p19; G_2D6ins_N | nos;nos | 35S;35S | - | - | For transient expression 2D6 with insertion for vacuolar targeting | A. t. EHA105 | ||
| pDGB2_Omega1R_YO37_YA04_p19_YA32_deltaTIPdmLSSmOrange | p19; deltaTIP_LssmOrange | nos;nos | 35S;35S | - | - | For transient expression Vacuolar gamma-TIP C-fused with mCherry | A. t. EHA105 | ||
| pDGB2_Omega1R_YO38_YA04_p19_YA40_2D6ins_vac_dmLSSmOrange | p19; 2D6ins_vac_dmLSSmOrange | nos;nos | 35S;35S | - | - | For transient expression 2D6 with insertion for vac targ fused with LssnOrange | A. t. EHA105 | ||
| pDGB2_Omega1R_YO39_YA04_p19_YA41_CTP_hNPRopt_GFP | p19; H_hNPRopt_N::GFP | nos;nos | 35S;35S | CTP | - | For transient expression Human NADPH P450 reductase (hNPR) opt C-fused with GFP with CTP | A. t. EHA105 | ||
| pDGB2_Omega1R_YO40_YA04_p19_YA42_CTP_ATR1var_GFP | p19; G_ATR1_GFP_N | nos;nos | 35S;35S | CTP | - | For transient expression A. thaliana AtR1 reductase with ins/del with CTP | A. t. EHA105 | ||
| pDGB2_Omega1R_YO41_YA04_p19_YA43_2D6ins_vac_mCherry | p19; 2D6ins_vac_mCherry | nos;nos | 35S;35S | - | - | For transient expression 2D6 with insertion for vac targ fused with mCherry | A. t. EHA105 | ||
| pDGB2_Omega1R_YO42_YA04_p19_YA44_2D6ins_vac_sGFP | p19; 2D6ins_vac_GFP | nos;nos | 35S;35S | - | - | For transient expression 2D6 with insertion for vac targ fused with GFP | A. t. EHA105 | ||
| pDGB2_Omega1R_YO43_YA04_p19_2D6sGFP | p19; 2D6sGFP | nos;nos | 35S;35S | - | - | For transient expression 2D6 fused with sGFP | A. t. EHA105 | ||
| pDGB2_Omega1R_YO44_YA04_p19_CTP2D6sGFP | p19; 2D6sGFP | nos;nos | 35S;35S | CTP | - | For transient expression 2D6 fused with sGFP, chloroplast targeting | A. t. EHA105 | ||
| pDGB2_Omega1R_YO45_YA04_p19_sGFP | p19; sGFP | nos;nos | 35S;35S | - | - | For transient expression sGFP | A. t. EHA105 | ||
| pDGB2_Omega1R_YO46_YA04_p19_CTPsGFP | p19; sGFP | nos;nos | 35S;35S | CTP | - | For transient expression sGFP, chloroplast targeting | A. t. EHA105 | ||
| pDGB2_Omega1R_YO47_YA04_p19_ YA50_G_2D6_M_mCherry_N | p19; 2D6mCherry | nos;nos | 35S;35S | CTP | - | For transient expression 2D6 fused with mCherry, chloroplast targeting | A. t. EHA105 | ||
| pDGB2_Omega1R_YO48_YA04_p19_ YA51_G_2D6_M_LssmOrange_N | p19; 2D6_LssmOrange | nos;nos | 35S;35S | CTP | - | For transient expression 2D6 fused with LssmOrange, chloroplast targeting | A. t. EHA105 | ||
| pDGB2_Omega1R_YO49_YA04_p19_ YA51_G_2D6_M_LicBM3_N | p19; 2D6_LicBM3 | nos;nos | 35S;35S | CTP | - | For transient expression 2D6 fused with LicBM3, chloroplast targeting | A. t. EHA105 | ||
| pDGB2_Omega1R_YO50_YA04_p19_YA57_1A2 | p19; 1A2 | nos;nos | 35S;35S | - | - | For transient expression 1A2 | A. t. EHA105 | ||
| pDGB2_Omega1R_YO51_YA04_p19_YA58_2D6GFPDeltaTIP | p19; 2D6GFPDeltaTIP | nos;nos | 35S;35S | - | - | For transient expression 2D6 fused with GFP and Delta TIP (C-terminus) | A. t. EHA105 | ||
| pDGB2_Omega1R_YO53_YA04_p19_YA60_GFPDeltaTIP | p19; GFPDeltaTIP | nos;nos | 35S;35S | - | - | For transient expression GFP fused with Delta TIP (C-terminus) | A. t. EHA105 | ||
| pDGB2_Omega1R_YO54_YA04_p19_YA61_DeltaTIPGFP | p19; DeltaTIPGFP | nos;nos | 35S;35S | - | - | For transient expression GFP fused with Delta TIP (N-terminus) | A. t. EHA105 | ||
| pDGB2_Omega1R_YO56_YA04_p19_YA62_AtWAK2_H_sGFP_M_HDEL | p19; sGFP | nos;nos | 35S;35S | ER_HDEL | - | sGFP fused with At ER signal sequence (N) and HDEL (C) | A. t. EHA105 | ||
| pDGB2_Omega1R_YO57_YA04_p19_ 3A4_M_sGFP | p19; 3A4_M_sGFP | nos;nos | 35S;35S | - | - | For transient expression 3A4 fused with GFP | A. t. EHA105 | ||
| pDGB2_Omega1R_YO58_YA56_cyb5_ cyb5R | cyb5; cyb5R | nos;nos | 35S;35S | - | - | For transient expression N.benthamiana cyb5 and cyb5 reductase in omega 1R vector | A. t. EHA105 | ||
| pDGB2_Omega1R_YO64_YA04_p19_ 1A2_M_sGFP | p19; 1A2_M_sGFP | nos;nos | 35S;35S | - | - | For transient expression 3A4 fused with GFP | A. t. EHA105 | ||
| pDGB2_Omega2_YO60_YA35_hNPR_YA04_p19 | hNPR; p19 | nos;nos | 35S;35S | - | - | For transient expression hNPR and p19 in omega 2 | A. t. EHA105 |
