2012-03-23T11:01:40Z VmGLT*(Gluc-Glucin/KeqGLT)/(KmGLTGLCo*(1+Gluc/KmGLTGLCo+Glucin/KmGLTGLCi+0.91*Gluc*Glucin/(KmGLTGLCi*KmGLTGLCo))) 2012-03-23T11:18:19Z VmGLK * (Glucin + ATP - (G6P * ADP) / KeqGLK) / (KmGLKATP * KmGLKGlucin * (1 + G6P/KmGLKG6P + Glucin/KmGLKGlucin) * (1 + ATP/KmGLKATP + ADP/KmGLKADP)) Vmax1*(Gluc/km1Gluc)*(PEP/km1PEP)/((1+Gluc/km1Gluc+G6P/km1G6P)*(1+PEP/km1PEP+PYR/km1PYR))/uVol Vmax2*(G6P/km2G6P)*(1-F6P/(G6P*Keq2))/(1+G6P/km2G6P+F6P/km2F6P)/uVol Vmax3*(1-PEP^n3PEP/(k3PEP^n3PEP+PEP^n3PEP))*1*(F6P^n3/km3F6P^n3)*(ATP/km3ATP)/((1+F6P^n3/km3F6P^n3+FBP/km3FBP)*(1+ATP/km3ATP+ADP/km3ADP))/uVol Vmax4*FBP/K4FBP*(1-G3P*DHAP/(FBP*Keq4))/(1+FBP/K4FBP+DHAP/K4DHAP+G3P/K4G3P+FBP*G3P/(K4FBP*Ki4G3P)+DHAP*G3P/(K4DHAP*K4G3P))/uVol Vmax5*(DHAP/km5DHAP)*(1-G3P/(DHAP*Keq5))/(1+DHAP/km5DHAP+G3P/km5G3P)/uVol Vmax6*(1-DPG*NADH/(G3P*NAD*P*Keq6))*(G3P/km6G3P)*(NAD/km6NAD)*(P/km6P)/((1+G3P/km6G3P+DPG/km6DPG)*(1+P/km6P)*(1+NAD/km6NAD+NADH/km6NADH))/uVol Vmax7*(DPG/km7DPG)*(ADP/km7ADP)*(1-P3G*ATP/(DPG*ADP*Keq7))/((1+DPG/km7DPG+P3G/km7P3G)*(1+ADP/km7ADP+ATP/km7ATP))/uVol Vmax8*(P3G/km8P3G)*(1-P2G/(P3G*Keq8))/(1+P3G/km8P3G+P2G/km8P2G)/uVol Vmax9*(P2G/km9P2G)*(1-PEP/(P2G*Keq9))/(1+P2G/km9P2G+PEP/km9PEP)/uVol Vmax10*(PEP/km10PEP)^n10*(ADP/km10ADP)*(1-PYR*ATP/(PEP*ADP*Keq10))/((1+(PEP/km10PEP)^n10+PYR/km10PYR)*(1+ADP/km10ADP+ATP/km10ATP))*(1/(1+P^3/(Ki10*(1+FBP/K10FBP+G6P/K10G6P)^3)))/uVol FBP/(FBP+k11FBP)*Vmax11*(PYR/km11PYR)*(NADH/km11NADH)*(1-Lac*NAD/(PYR*NADH*Keq11))/((1+PYR/km11PYR+Lac/km11Lac)*(1+NADH/km11NADH+NAD/km11NAD))/uVol Vmax12*(PYR/K12PYR)*(1-ACLAC/(PYR*Keq12))*(PYR/K12PYR+ACLAC/K12ACLAC)^(h12-1)/(1+(PYR/K12PYR+ACLAC/K12ACLAC)^h12)/uVol Vmax13*ACLAC/Km13ACLAC*(1-ACET/(ACLAC*Keq13))*(ACLAC/Km13ACLAC+ACET/Km13ACET)^(h13-1)/(1+(ACLAC/Km13ACLAC+ACET/Km13ACET)^h13)/uVol Vmax14*(ACET/Km14ACET)/(1+ACET/Km14ACET)/uVol Vmax15*ACLAC/uVol Vmax16*(ACET/km16ACET)*(NADH/km16NADH)*(1-BUT*NAD/(ACET*NADH*Keq16))/((1+ACET/km16ACET+BUT/km16BUT)*(1+NADH/km16NADH+NAD/km16NAD))/uVol Vmax17*(PYR/km17PYR)*(COA/km17COA)*(1-FR*ACCOA/(PYR*COA*Keq17))/((1+(G3P+DHAP)/ki17)*(1+PYR/km17PYR+FR/km17FOR)*(1+COA/km17COA+ACCOA/km17ACCOA))/uVol Vmax18*(ACCOA/km18ACCOA)*(P/km18P)*(1-ACP*COA/(ACCOA*P*Keq18))/((1+ACCOA/km18ACCOA+ACP/km18ACP)*(1+P/km18P+COA/km18COA))/uVol Vmax19*(ACP/km19ACP)*(ADP/km19ADP)*(1-AC*ATP/(ACP*ADP*Keq19))/((1+ACP/km19ACP+AC/km19AC)*(1+ADP/km19ADP+ATP/km19ATP))/uVol Vmax20*(PYR/k20PYR)*(NAD/k20NAD)*(COA/k20COA)/((1+PYR/k20PYR)*(1+NAD/k20NAD+NADH/k20NADH)*(1+COA/k20COA+ACCOA/k20ACCOA))*(1/(1+Ki20*(NADH/NAD)))/uVol (Vmax21*NADH*ACCOA/(K21NADH*K21ACCOA)-Vmax21*NAD*COA*ACAL/(K21NADH*K21ACCOA*KEQ21))/((1+NADH/K21NADH+NAD/K21NAD)*(1+ACCOA/K21ACCOA+COA/K21COA)*(1+ACAL/K21ACAL))/uVol Vmax22*(ACAL/km22ACAL)*(NADH/km22NADH)*(1-ETOH*NAD/(ACAL*NADH*Keq22))/((1+ACAL/km22ACAL+ETOH/km22ETOH)*(1+NADH/km22NADH+NAD/km22NAD))/uVol Vmax23*(ATP/ADP)^N23/(K23ATP^N23+(ATP/ADP)^N23)/uVol Vmax24*NADH/KM24OXNADH*O2/KM24OXO/((1+NADH/KM24OXNADH+NAD/KM24OXNAD)*(1+O2/KM24OXO))/uVol Vmax25*(FBP/km25FBP)/(1+FBP/km25FBP+F6P/km25F6P)/uVol Vmax26*(G6P/K26G6P)/(1+G6P/K26G6P)/uVol Vmax27*(G6P/km27G6P)*(1-G1P/(G6P*Keq27))/(1+G6P/km27G6P+G1P/km27G1P)/uVol Vmax28*(G1P/k28g1p)*(ATP/k28atp)*(NADH/k28nadh)/((1+G1P/k28g1p+P/k28p)*(1+ATP/k28atp+ADP/k28ADP)*(1+NADH/k28nadh+NAD/k28nad))/uVol Vmax29*(Glucin/km29Glucin)*(ATP/km29ATP)/((1+Glucin/km29Glucin+G6P/km29G6P)*(1+ATP/km29ATP+ADP/km29ADP))/uVol 2013-01-09T13:44:19Z

The principles of Stealthy Engineering (Adamczyk et al.: Biotechnology Journal 2012; 7(7):877-83) are illustrated in this model by emulating a cross engineering intervention between L. lactis and S. cerevisiae.

The case study consists of replacing the native glucose uptake system of L. lactis with that native to the yeast S. cerevisiae. A modified version of Hoefnagel et al.’s model of L. lacrtis’ central metabolism was used as starting point. The total functional replacement of the PTS with the yeast glucose transport plus hexokinase was achieved by modifying the relevant parameters according to the Stealthy Engineering approach discussed in Adamczyc et al. 2012.

2013-01-09T13:49:22Z 2013-01-09T13:52:35Z 2013-01-09T13:49:22Z 2013-01-09T13:51:45Z 2013-01-09T13:51:57Z 2013-01-09T13:49:33Z 2013-01-09T13:55:04Z 2013-01-09T13:55:21Z 2013-01-09T13:46:05Z 2013-01-09T13:49:45Z 0 1.194214333029262e+25 2.103111972233066e+23 4.68361379669891e+24 3.175607500414705e+24 1.571785649884442e+24 1.073811209476329e+19 1.423736985904262e+23 5.987141569361228e+23 3.696944167597619e+23 3.744833520494124e+23 6.02214179e+23 3.198014674477526e+24 1.488981927959078e+24 5.685062105332819e+19 7.052758914292798e+22 2.284900664372231e+20 1.468047404871458e+21 9.992499840994631e+22 2.045676727830598e+25 3.150783422375889e+22 1.338527993301936e+24 3.500022062298871e+21 1.666811931025939e+23 1.204428358e+24 6.02214179e+22 6.02214179e+22 0 1.204428358e+22 1.204428358e+22 0 6.02214179e+22 6.02214179e+22 6.02214179e+22 6.02214179e+18 1 Automatically generated report.