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    "experiment_type": "Luciferase exp",
    "id": "3647",
    "status": "finished",
    "name": "cca1 lhy Long #1 CAB2 marker",
    "purpose": "Test for period changes during seedlling growth in the TopCount, in WT and cca lhy double mutants",
    "description": "If periods change during development, then our genetic screens for preiod-altering clock mutants might recover developmental mutants. Leaf movement assays suggestted that period might change. Ten-day data collections in the TopCount were intended to test for such period changes using luciferase. The lhy cca1 mutant was of particular interest, as it had a very short period to start with - was this perhaps rescued in older plants? In fact, ultradian rhythms were revealed..",
    "comments": "Data collection by Victoria Hibberd ca. September 2003; files found by Andrew Millar in 2012, reanalysed on sabbatical in 2015.",
    "local_files": "C:\\Users\\Andrew\\Documents\\Data\\LUC\\Vicky; C:\\Users\\Andrew\\Archives\\ex-Warwick 250MB HDD-1\\Q Drive\\VickyH;",
    "provenance": {
      "site": "Warick",
      "author": "Victoria Hibberd",
      "start_date": "2003-09-01"
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      "name": "12L:12D WL",
      "medium": "Agar 1.5% MS 1 3% Sucrose",
      "incubation": "2 days at 4C",
      "growth_space": "Sanyo",
      "duration": "6.0",
      "comment": "Standard Warwick procedure would be described in (e.g.) Hall et al. TIC paper Plant Cell 2003",
      "temperature_conditions": {
        "label": "const. 22.0C",
        "env_conditions": {
          "type": "constant_temp",
          "label": "const. 22.0C",
          "duration": "6",
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            "duration": "6",
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            "base_value": "22.0",
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          "spectrum": "white",
          "intensity": "80",
          "source": "tube",
          "env_conditions": {
            "type": "diurnal_light",
            "label": "LD 12:00:12:00",
            "duration": "6",
            "env_day": {
              "type": "diurnal_light",
              "label": "diurnal light",
              "duration": "6",
              "cycle_length": "24",
              "base_value": "0.0",
              "second_value": "100.0",
              "env_period": {
                "start_time": "0:00",
                "duration": "12:00"
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            }
          }
        }
      }
    },
    "experimental_condition": {
      "name": "constant R+B",
      "medium": "Agar 1.5% MS 1 3% Sucrose",
      "growth_space": "equipment compartment",
      "duration": "11.0",
      "comment": "again, standard Warwick procedure as described in Hall et al. Plant Cell 2003. However, light levels in the black wells in the TopCount were very low. 2uE each of R+B is the maximum. R and B LEDs were in alternating rows of colour, next to the stackers. LEDs at each end of the plate (i.e. next to well columns 1 and 12) and would be low (1-2 micromol/m2/sec) and uneven (ca. half in rows 6,7) despite the mirror plates.",
      "temperature_conditions": {
        "label": "const. 22.0C",
        "env_conditions": {
          "type": "constant_temp",
          "label": "const. 22.0C",
          "duration": "11",
          "env_day": {
            "type": "constant_temp",
            "label": "constant temperature",
            "duration": "11",
            "cycle_length": "24",
            "base_value": "22.0",
            "second_value": "22.0"
          }
        }
      },
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        "label": "B+R: LL",
        "light_channel": [
          {
            "spectrum": "red",
            "intensity": "2",
            "source": "LED",
            "env_conditions": {
              "type": "constant_light",
              "label": "LL",
              "duration": "11",
              "env_day": {
                "type": "constant_light",
                "label": "constant light",
                "duration": "11",
                "cycle_length": "24",
                "base_value": "0.0",
                "second_value": "100.0",
                "env_period": {
                  "start_time": "0:00",
                  "duration": "24:00"
                }
              }
            }
          },
          {
            "spectrum": "blue",
            "intensity": "2",
            "source": "LED",
            "env_conditions": {
              "type": "constant_light",
              "label": "LL",
              "duration": "11",
              "env_day": {
                "type": "constant_light",
                "label": "constant light",
                "duration": "11",
                "cycle_length": "24",
                "base_value": "0.0",
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                "env_period": {
                  "start_time": "0:00",
                  "duration": "24:00"
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    "measurement": {
      "technique": "Luciferase luminescence",
      "equipment": "Top count 1",
      "parameters": "Each plate held 5 mins in dark before counting started. Counting with 2-detector TopCount took ca. 2.8 mins per plate. Each plate counted every 29.01 minutes (XL trendline is actually 29.8 mins, but no matter)."
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          "experiment_cond_name": "constant R+B",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "92",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "cca1;lhy",
            "line": "314",
            "provenance": "Robust stock"
          },
          "marker": "CAB",
          "growth_cond_name": "12L:12D WL",
          "experiment_cond_name": "constant R+B",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "93",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "cca1;lhy",
            "line": "314",
            "provenance": "Robust stock"
          },
          "marker": "CAB",
          "growth_cond_name": "12L:12D WL",
          "experiment_cond_name": "constant R+B",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "94",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "cca1;lhy",
            "line": "314",
            "provenance": "Robust stock"
          },
          "marker": "CAB",
          "growth_cond_name": "12L:12D WL",
          "experiment_cond_name": "constant R+B",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "95",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "cca1;lhy",
            "line": "314",
            "provenance": "Robust stock"
          },
          "marker": "CAB",
          "growth_cond_name": "12L:12D WL",
          "experiment_cond_name": "constant R+B",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "96",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "cca1;lhy",
            "line": "314",
            "provenance": "Robust stock"
          },
          "marker": "CAB",
          "growth_cond_name": "12L:12D WL",
          "experiment_cond_name": "constant R+B",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        }
      ]
    }
  },
  "pedro": {},
  "biblio": {
    "principal": "Andrew Millar",
    "institution": "University of Warwick University of Edinburgh",
    "author": [
      "Victoria Hibberd",
      "Anthony Hall"
    ],
    "curator": "Andrew Millar"
  }
}