| Property | Value |
|---|---|
| BioDare ID | 13227547204752 |
| Author | Ito S |
| Institution | Nagoya University |
| License | CC_BY |
PRR9 mRNA and protein were measured under the same conditions. This file has PRR9 protein only.
This PRR9 protein profile rises more slowly than the FLAG-tagged PRR9 protein in Nakamichi Plant Cell 2010.
Plants were ground to a fine powder in liquid nitrogen. Crude protein extracts were prepared in 2 vols. of SDS loading buffer (2% SDS, 175 mM Tris–HCl pH 8.0, 0.6 mM EDTA pH 8.0, 3.2% 2-mercaptoethanol, 25% glycerol, 0.008% bromophenol blue). Extracts were immediately boiled for 10 min and the supernatant collected after centrifugation at 6,000×g for 10 min.
Western blot (TAP-tagged protein)
Literature data from: '' by: .
These protein samples were separated by 10% SDS–PAGE and transferred onto a PVDF membrane (for each examination, 80 μg of total protein was used). The protein A domains of the TAP-tag were detected with a PAP (peroxidase–anti-peroxidase) antibody (Sigma). Immunoreactive bands were detected by the ECL plus Western blotting system (Amersham Pharmacia Biotech, USA).
Growth on not entered for 10.0 days (LD standard).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 10 | 24 | 0:00 | 12:00 | white | LED | 70 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 10 | 24 | 20 | -- | -- | -- |
Growth on not entered for 1.0 days (12:12).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 1 | 24 | 0:00 | 12:00 | white | LED | 40 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 1 | 24 | 20 | -- | -- | -- |