| Property | Value |
|---|---|
| BioDare ID | 13558463723152 |
| Author | Sarah Hodge |
| Institution | University of Edinburgh |
| License | CC_BY |
LUC imaging10:10 blue+red into constant light
testing acute light responses at dawn and dusk
sprayed upon transfer from entrainment
Luciferase luminescence (HAM 2)
imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture
Growth on Agar 1.5% MS 0.5 3% Sucrose for 7.0 days (10L:10D).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 7 | 20 | 0:00 | 10:00 | white | tube | 40 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 20 | 22 | -- | -- | -- |
Growth on Agar 1.5% MS 0.5 3% Sucrose for 7.0 days (4x10L:10D into LL). Imaging was started 10 mins late at 10.05am.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 20 | 0:00 | 10:00 | blue | LED | 40 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 40 |
| diurnal light | 4 | 20 | 0:00 | 10:00 | red | LED | 40 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 40 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 20 | 22 | -- | -- | -- |