Mizoguchi2002 LHY CCA1 redundancy

Property Value
BioDare ID 13228410657846
Author Mizoguchi Tsuyoshi
Institution John Innes Centre
License CC_BY

Description

Literature data from: 'LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis' by: Mizoguchi Tsuyoshi.

    lhy/cca1, lhy, cca1 mutant rhythms were extensively characterized in this paper (lhy-12 in Ler; cca1-1 in ws)

Purpose

LL time courses for TOC1, GI, LHYL1, COL1, CCR2, LHY, CCA1 mRNA for wt, lhy, cca1, lhy/cca1; soil

Comments

Sample preparation

Northern blot

Measurement

Protocol

Northern Blot ()

Experimental conditions

soil

Growth on Soil for 8.0 days (soil). temperature, light quality see Putterill, J., Robson, F., Lee, K., Simon, R., and Coupland, G. (1995). The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80, 847–857

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 8 24 0:00 18:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 8 24 20 -- -- --

soil LL

Growth on Soil for 3.0 days (soil LL). temperature, light quality see Putterill, J., Robson, F., Lee, K., Simon, R., and Coupland, G. (1995). The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80, 847–857

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 3 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 20 -- -- --

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