Mizoguchi2002_wt_lhycca1_16L8D_TOC1_GI_LHYL1_COL1

Property Value
BioDare ID 13228375909917
Author Mizoguchi Tsuyoshi
Institution John Innes Centre
License CC_BY

Description

Literature data from: 'LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis' by: Mizoguchi Tsuyoshi.

    lhy/cca1, lhy, cca1 mutant rhythms were extensively characterized in this paper (lhy-12 in Ler; cca1-1 in ws)

Purpose

good quality 16:8 data, soil for TOC1, GI, LHYL1, COL1 mRNA

Comments

Sample preparation

Northern blot

Measurement

Protocol

Northern Blot ()

Experimental conditions

soil

Growth on Soil for 8.0 days (soil). temperature, light quality see Putterill, J., Robson, F., Lee, K., Simon, R., and Coupland, G. (1995). The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80, 847–857

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 8 24 0:00 18:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 8 24 20 -- -- --

soil 18:6

Growth on Soil for 2.0 days (soil 18:6). temperature, light quality see Putterill, J., Robson, F., Lee, K., Simon, R., and Coupland, G. (1995). The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80, 847–857

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 2 24 0:00 18:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 2 24 20 -- -- --

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