12C RL-cryptochrome paper-rep2

Property Value
BioDare ID 13390805417188
Author Peter Gould
Institution University of Liverpool
License CC_BY

Description

12C, RL, on sucrose

Purpose

Temperature-comp-photoreceptor mutants

Comments

Sample preparation

spray 24h prior with 5mM LUC

Measurement

Protocol

Luciferase luminescence (HAM 1)

2x2 bin 20 min exposure

Experimental conditions

22C LD

Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (22C LD).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 7 24 0:00 12:00 white tube 80

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

12C RL

Growth on Agar 1.5% MS 1 3% Sucrose for 5.0 days (12C RL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 5 24 0:00 24:00 red LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 5 24 12 -- -- --

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