| Property | Value |
|---|---|
| BioDare ID | 13185871416482 |
| Author | Peter Gould |
| Institution | |
| License | CC_BY |
BL 17C 2LD to 5LL
To determine the effect of mutants on circadian clock in a temperature specific manner. RoBuST mutant library.
Part of a set of data from all RoBuST mutants looking at their effects on clock over a range of environmental conditions from RL, BL, RBL at 12, 17 and 27C
the day before spray with 5uM LUC
Luciferase luminescence (HAM 2)
20 minute exposure 2x2 bin every 2 hours
Growth on Agar 1.5% MS 1 for 7.0 days (Liv Growth).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 7 | 24 | 0:00 | 12:00 | white | tube | 80 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.5% MS 1 for 7.0 days (B30 2LD to 5LL).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 2 | 24 | 0:00 | 12:00 | blue | LED | 30 |
| constant light | 5 | 24 | 0:00 | 24:00 | blue | LED | 30 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 17 | -- | -- | -- |