1xFRD, 4xcFR, 4xDD

Property Value
BioDare ID 12730590368619
Author Laszlo Kozma-Bognar
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 12:12 Far Red into constant FR and DD

Purpose

testing clock response to Far Red light

Comments

Not clear if regions are around 1 or 2 seedlings, considered only one seedling

Sample preparation

sprayed 24 hours before imaging

Measurement

Protocol

Luciferase luminescence (HAM 2)

Start at ZT1:09, a picture avery 2 hours

imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture

Experimental conditions

FRD 12:12

Growth on Agar 1.5% MS 1 3% Sucrose for 5.0 days (FRD 12:12). entrainment

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 5 24 0:00 12:00 infra red LED 8

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 5 24 22 -- -- --

1xFRD, 4xcFR, 4xDD

Growth on Agar 1.5% MS 1 3% Sucrose for 9.0 days (1xFRD, 4xcFR, 4xDD). 1 day 12/12 far red, 4 days constant far red, 4 days constant dark

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 12:00 infra red LED 8
constant light 4 24 0:00 24:00 infra red LED 8
constant dark 4 24 -- -- infra red LED 8

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 9 24 22 -- -- --

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