AT0032: 4x15:9-LL

Property Value
BioDare ID 12730755364126
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

long day photoperiod experiment

Purpose

photoperiod experiment

Comments

Sample preparation

sprayed upon transfer from entrainment

Measurement

Protocol

Luciferase luminescence (HAM 3)

imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture

Experimental conditions

LD15:9 22c

Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD15:9 22c). entrainment

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 6 24 0:00 15:00 white tube 100

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 6 24 22 -- -- --

AT0032: 4x15:9-LL

Growth on Agar 1.5% MS 1 3% Sucrose for 8.0 days (AT0032: 4x15:9-LL). first day not imaging, 4 days long day, then constant white light

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 4 24 0:00 15:00 red LED 10
constant light 4 24 0:00 24:00 red LED 10
diurnal light 4 24 0:00 15:00 blue LED 10
constant light 4 24 0:00 24:00 blue LED 10

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 8 24 22 -- -- --

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