AT0039: 6:18 then DD

Property Value
BioDare ID 12730747977016
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

6:18 then DD

Purpose

6:18 then DD

Comments

-infection -PRR9 groups died

Sample preparation

sprayed upon transfer from entrainment

Measurement

Protocol

Luciferase luminescence (HAM 3)

imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture

Experimental conditions

LD6:18 22c

Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD6:18 22c). entrainment

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 6 24 0:00 6:00 white tube 100

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 6 24 22 -- -- --

AT00392: 6:18 then DD

Growth on Agar 1.5% MS 1 3% Sucrose for 8.0 days (AT00392: 6:18 then DD). first day not imaging, 4 days short day, then constant dark

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 4 24 0:00 6:00 red LED 10
constant dark 1 24 -- -- red LED 10
diurnal light 4 24 0:00 6:00 blue LED 10
constant dark 4 24 -- -- blue LED 10

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 8 24 22 -- -- --

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