| Property | Value |
|---|---|
| BioDare ID | 12730739172368 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
LUC imaging 3:21 LD to LL in RB light
testing different photoperiod effect on entrainment and phase on release to LL
Plates wrong way round! contamination on cca1 lhy gi CCR2 & cca1lhy gi CCR2 plate
sprayed upon transfer from entrainment
Luciferase luminescence (HAM 1)
imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture
Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD3:21 22c). entrainment
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 3:00 | white | tube | 100 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (4x3:21-LL). first day not imaging, 4 days of LD 3:21, 3 days LL P13H1
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| cyclic light pulses | 4 | 24 | 00:00 | 03:00 | red | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 10 |
| cyclic light pulses | 4 | 24 | 00:00 | 03:00 | blue | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 10 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |