| Property | Value |
|---|---|
| BioDare ID | 12730738745576 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
4 days of 12hrs light, 12hrs dark into 3 days constant light
changes to photoperiod
sprayed upon transfer from entrainment
Luciferase luminescence (HAM 1)
imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture
Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD 4x 12:12-LL 22c). entrainment
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 12:00 | white | tube | 100 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (AT0011 4x 12:12-LL). Day 1, 12:12, Day2 12:12, Day3 12:12, Day4 12:12, Day5 LL, Day6 LL, Day7 LL
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 0:00 | 12:00 | blue | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 10 |
| diurnal light | 4 | 24 | 0:00 | 12:00 | red | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 10 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |