| Property | Value |
|---|---|
| BioDare ID | 12730736018318 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
LUC imaging 18:6 LD to LL in RB light
testing different photoperiod effect on entrainment and phase on release to LL
CCR2 in lhycca1 and lhy cca1 gi are contaminated.
sprayed upon transfer from entrainment
Luciferase luminescence (HAM 1)
imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture
Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD18:6 22c). entrainment
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 18:00 | white | tube | 100 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (4x18:6-LL). first day not imaging, 4 days of LD 18:6 P13H1
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| cyclic light pulses | 4 | 24 | 00:00 | 18:00 | red | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 10 |
| cyclic light pulses | 4 | 24 | 00:00 | 18:00 | blue | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 10 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |