AT0006 4x LD-3xLL

Property Value
BioDare ID 12730734647405
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

4 days LD followed by 3 days constant light

Purpose

changes to photoperiod

Comments

Sample preparation

sprayed upon transfer from entrainment

Measurement

Protocol

Luciferase luminescence (HAM 1)

imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture

Experimental conditions

LD18:6 22c

Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD18:6 22c). entrainment

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 6 24 0:00 18:00 white tube 100

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 6 24 22 -- -- --

AT0006 4x LD-3xLL

Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (AT0006 4x LD-3xLL). 4 days 18L:6D, followed by 3 days at constant light

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 4 24 0:00 18:00 blue LED 10
constant light 3 24 0:00 24:00 blue LED 10
diurnal light 4 24 0:00 18:00 red LED 10
constant light 3 24 0:00 24:00 red LED 10

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

Files