AT0005 4x SD-3xLL

Property Value
BioDare ID 12730734291594
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

alternate 4 days SD followed by 3 days constant light

Purpose

changes to photoperiod

Comments

Sample preparation

sprayed upon transfer from entrainment

Measurement

Protocol

Luciferase luminescence (HAM 1)

imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture

Experimental conditions

LD 6:18 22c

Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD 6:18 22c). entrainment

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 6 24 0:00 6:00 white tube 100

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 6 24 22 -- -- --

AT0005 4x SD-3xLL

Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (AT0005 4x SD-3xLL). Day 1, SD, Day2 SD, Day3 SD, Day4 SD, Day5 LL, Day6 LL, Day7 LL

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 4 24 0:00 6:00 blue LED 10
constant light 3 24 0:00 24:00 blue LED 10
diurnal light 4 24 0:00 6:00 red LED 10
constant light 3 24 0:00 24:00 red LED 10

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

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