| Property | Value |
|---|---|
| BioDare ID | 12730555174222 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
LUC imaging 3:21 blue+red into constant light, TOC1 data show anomalous dawn peak, so TOC1 data not used for phase analysis in Edwards et al. 2010
testing circadian phase across photoperiods
sprayed upon transfer from entrainment
Luciferase luminescence (HAM 1)
imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture
Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD 3:21). entrainment
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 3:00 | white | tube | 100 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (4x 3:21-LL). first day not imaging, 4 days 3/21 blue red, 3 days constant red blue P1H1
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 0:00 | 3:00 | blue | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 10 |
| diurnal light | 4 | 24 | 0:00 | 3:00 | red | LED | 10 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 10 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |