| Property | Value |
|---|---|
| BioDare ID | 12729820597730 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
LUC imaging 15L:9D into constant dark
testing phase changes of clock components under different photoperiods
microwave seed sterilisation
sprayed upon transfer from entrainment in Sanyo chamber to Percival cabinet
Luciferase luminescence (HAM 2)
Standard Adrian Thomson protocol for imaging
imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture
Growth on Agar 1.5% MS 1 3% Sucrose for 6.0 days (LD15:09 22c). entrainment
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 15:00 | white | tube | 100 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.5% MS 1 3% Sucrose for 7.0 days (4x15:9-DD). first day not imaging, 4 days photoperiod, 3 days constant dark P13H2 (Percival chamber number 13; Hamamatsu camera 2)
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 0:00 | 15:00 | blue | LED | 10 |
| constant dark | 3 | 24 | -- | -- | blue | LED | 10 |
| diurnal light | 4 | 24 | 0:00 | 15:00 | red | LED | 10 |
| constant dark | 3 | 24 | -- | -- | red | LED | 10 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |