| Property | Value |
|---|---|
| BioDare ID | 12730680847492 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD
Analysis of CCA1 and CCR2 in phyb, elf3-4, elf4-1 and toc1-4 mutants under strict FR light treatment
Extremely bad contamination (fungus and bacteria)
Plates were sprayed with luciferin 24 hrs before measurement
Luciferase luminescence (HAM 3)
A picture every 1.5 hr
Growth on Agar 1.2% MS 0.5;Agar 1.5% MS 1 3% Sucrose for 4.0 days (FR growth conditions).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| constant dark | 1 | 24 | -- | -- | infra red | LED | 2 |
| diurnal light | 3 | 24 | 0:00 | 12:00 | infra red | LED | 2 |
| diurnal light | 1 | 24 | 0:00 | 3:00 | white | LED | 80 |
| constant dark | 3 | 24 | -- | -- | white | LED | 80 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 4 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5;Agar 1.5% MS 1 3% Sucrose for 6.0 days (FR experimental conditions).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 1 | 24 | 0:00 | 12:00 | infra red | LED | 2 |
| constant light | 3 | 24 | 0:00 | 24:00 | infra red | LED | 2 |
| constant dark | 2 | 24 | -- | -- | infra red | LED | 2 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |