| Property | Value |
|---|---|
| BioDare ID | 12725464084711 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD
Analysis of the main clock markers in elf4, elf3, gi and phya mutants under strict FR light treatment
Plates were sprayed with luciferin 24 hrs before measurement
Luciferase luminescence (HAM 3)
A picture every 1.5 hr
Growth on Agar 1.2% MS 0.5;Agar 1.5% MS 1 3% Sucrose for 4.0 days (FR growth conditions).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| constant dark | 1 | 24 | -- | -- | infra red | LED | 2 |
| diurnal light | 3 | 24 | 0:00 | 12:00 | infra red | LED | 2 |
| diurnal light | 1 | 24 | 0:00 | 3:00 | white | LED | 80 |
| constant dark | 3 | 24 | -- | -- | white | LED | 80 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 4 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5;Agar 1.5% MS 1 3% Sucrose for 6.0 days (FR experimental conditions).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 1 | 24 | 0:00 | 12:00 | infra red | LED | 2 |
| constant light | 3 | 24 | 0:00 | 24:00 | infra red | LED | 2 |
| constant dark | 2 | 24 | -- | -- | infra red | LED | 2 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |