Analysis of Control Strains Neurospora crassa
| Property |
Value |
| BioDare ID |
13945617808900 |
| Author |
Felipe Munoz |
| Institution |
Pontificia Universidad Católica de Chile |
| License |
CC_BY |
Description
Purpose
Compare different siblings of the parental/control strains in the global screening of transcription factors capable to affect the circadian oscilator
Comments
Sample preparation
Measurement
Protocol
Luciferase luminescence (CCD Camera)
Experimental conditions
LL Condition
Growth on LNN-CCD for 1.0 days (LL Condition).
Light
| Type |
Duration (days) |
Cycle (h) |
Start |
Duration |
Spectrum |
Source |
Intensity |
| constant light |
1 |
24 |
0:00 |
24:00 |
white |
tube |
70 |
Temperature
| Type |
Duration (days) |
Cycle (h) |
Base (°C) |
Warm (°C) |
Warm start |
Warm duration |
| constant temperature |
1 |
24 |
25 |
-- |
-- |
-- |
Free running
Growth on LNN-CCD for 5.0 days (Free running).
Light
| Type |
Duration (days) |
Cycle (h) |
Start |
Duration |
Spectrum |
Source |
Intensity |
| constant dark |
5 |
24 |
-- |
-- |
white |
tube |
75 |
Temperature
| Type |
Duration (days) |
Cycle (h) |
Base (°C) |
Warm (°C) |
Warm start |
Warm duration |
| constant temperature |
5 |
24 |
25 |
-- |
-- |
-- |
Files
- Controls.3.xlsx
- STANDARD_RAWDATAFILE.3.txt
- STANDARD_DATAFILE.3.txt