Carbon Source

Property Value
BioDare ID 1181
Author Felipe Munoz
Institution Pontificia Universidad Católica de Chile
License CC_BY

Description

Purpose

Compare Control Strains in different Carbon Sources

Comments

Sample preparation

Measurement

Protocol

Luciferase luminescence (CCD Camera)

Experimental conditions

LL

Growth on LNN-CCD for 1.0 days (LL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 1 24 0:00 24:00 white tube 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 1 24 25 -- -- --

LL2

Growth on LNN-CCD 2% G for 1.0 days (LL2).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 1 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 1 24 25 -- -- --

LL3

Growth on Avicel 2% for 1.0 days (LL3).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 1 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 1 24 25 -- -- --

LL4

Growth on Avicel 0.03% for 1.0 days (LL4).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 1 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 1 24 25 -- -- --

LL5

Growth on Miscanthus 2% for 1.0 days (LL5).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 1 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 1 24 25 -- -- --

Free Running

Growth on LNN-CCD for 5.0 days (Free Running).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant dark 5 24 -- -- white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 5 24 25 -- -- --

Free Running 2

Growth on LNN-CCD 2% G for 4.0 days (Free Running 2).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 4 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 4 24 25 -- -- --

Free Running 3

Growth on Avicel 2% for 4.0 days (Free Running 3).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 4 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 4 24 25 -- -- --

Free Running 4

Growth on Avicel 0.03% for 4.0 days (Free Running 4).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 4 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 4 24 25 -- -- --

Free Running 5

Growth on Miscanthus 2% for 4.0 days (Free Running 5).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 4 24 0:00 24:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 4 24 25 -- -- --

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