Entrainment Control Analysis

Property Value
BioDare ID 1178
Author Felipe Munoz
Institution Pontificia Universidad Católica de Chile
License CC_BY

Description

Purpose

Compare Period Calculations using entrainment and non entrainment strains for 72 hours

Comments

Sample preparation

Measurement

Protocol

Luciferase luminescence (CCD Camera)

Experimental conditions

LL

Growth on LNN-CCD for 3.0 days (LL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant light 3 24 0:00 24:00 white tube 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 25 -- -- --

LD

Growth on LNN-CCD for 3.0 days (LD).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 3 24 0:00 12:00 white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 25 -- -- --

Free Running

Growth on LNN-CCD for 5.0 days (Free Running).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
constant dark 5 24 -- -- white LED 40

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 5 24 25 -- -- --

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