| Property | Value |
|---|---|
| BioDare ID | 2630 |
| Author | Yvette Wilson |
| Institution | |
| License |
Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Partner experiment: 080115H1
Plants sprayed with luciferin on day 7 of growth conditions and transferred to experimental conditions. Imaging was started on day 8.
Luciferase luminescence (HAM 2)
Seedlings were treated on 6th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d7.
Imaging script started 5 minutes before ZT0 55mins wait, 5mins lights off, 30mins capture
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12D). Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 7 | 24 | 0:00 | 12:00 | white | LED | 90 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12D x4 LLx3). At max light intensity red = 51 umolm-2s-1 and blue light intensity is 32 umol-2s-1.
plants were transferred to experimental condiditons at ZT3
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 0:00 | 12:00 | red | LED | 51 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 51 |
| diurnal light | 4 | 24 | 0:00 | 12:00 | blue | LED | 32 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 32 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |