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"experiment_type": "Luciferase exp", "id": "1925", "status": "in progress", "name": "210814H1", "purpose": "Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock", "description": "Plants entrained in 12L 12D white light.\n\nExperimental conditions (Red and Blue)\n12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, \nDawn is at ZT0 and dusk (shady dark) is at ZT12.", "comments": "Pedro is not able to record changes in intensity during an experiment, so please refer to the description & experimental conditions comment for true experimental conditions.\n\nPartner Experiment: 210814H2\n\nImages binned 2x2 to obtain signal from prr7prr9 CCR2:LUC line", "provenance": { "site": "Edinburgh", "author": "Yvette Wilson", "start_date": "2014-08-20" }, "growth_condition": { "name": "12L:12D", "medium": "Agar 1.2% MS 0.5", "incubation": "7 days at 4C", "growth_space": "Percival", "duration": "9.0", "comment": "Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.", "temperature_conditions": { "label": "const. 22.0C", "env_conditions": { "type": "constant_temp", "label": "const. 22.0C", "duration": "9", "env_day": { "type": "constant_temp", "label": "constant temperature", "duration": "9", "cycle_length": "24", "base_value": "22.0", "second_value": "22.0" } } }, "light_conditions": { "label": "W: LD 12:00:12:00", "light_channel": { "spectrum": "white", "intensity": "90", "source": "tube", "env_conditions": { "type": "diurnal_light", "label": "LD 12:00:12:00", "duration": "9", "env_day": { "type": "diurnal_light", "label": "diurnal light", "duration": "9", "cycle_length": "24", "base_value": "0.0", "second_value": "100.0", "env_period": { "start_time": "0:00", "duration": "12:00" } } } } } }, "experimental_condition": { "name": "12L:12ShDx7", "medium": "Agar 1.2% MS 0.5", "growth_space": "Percival + LED", "duration": "7.0", "comment": "12 hours of bright light (90 umolm-2s-2) followed by 12 hours of 'shady dark' (6 umolm-2s-2) for 7 days\n\nThe first dawn starts at ZT0\n\nThe light intensities are controlled by the arduino.\n\nAt max light intensity red = 51 umolm-2s-1 and blue light intensity is 32 umol-2s-1.\n\nplants were transferred to experimental condiditons at ZT3", "temperature_conditions": { "label": "const. 22.0C", "env_conditions": { "type": "constant_temp", "label": "const. 22.0C", "duration": "7", "env_day": { "type": "constant_temp", "label": "constant temperature", "duration": "7", "cycle_length": "24", "base_value": "22.0", "second_value": "22.0" } } }, "light_conditions": { "label": "B+R: LD 12:00:12:00", "light_channel": [ { "spectrum": "blue", "intensity": "32", "source": "LED", "env_conditions": { "type": "diurnal_light", "label": "LD 12:00:12:00", "duration": "7", "env_day": { "type": "diurnal_light", "label": "diurnal light", "duration": "7", "cycle_length": "24", "base_value": "0.0", "second_value": "100.0", "env_period": { "start_time": "0:00", "duration": "12:00" } } } }, { "spectrum": "red", "intensity": "51", "source": "LED", "env_conditions": { "type": "diurnal_light", "label": "LD 12:00:12:00", "duration": "7", "env_day": { "type": "diurnal_light", "label": "diurnal light", "duration": "7", "cycle_length": "24", "base_value": "0.0", "second_value": "100.0", "env_period": { "start_time": "0:00", "duration": "12:00" } } } } ] } }, "measurement": { "technique": "Luciferase luminescence", "equipment": "HAM 1", "parameters": "Imaging script started 1 hr 25 minutes after ZT0\n55mins wait, 5mins lights off, 30mins capture", "description": "Seedlings were treated on 9th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d10." }, "sample_preparation": { "method": "Plants sprayed with luciferin on day 9 of growth conditions and transferred to experimental conditions. Imaging was started on day 10." }, "samples": { "sample": [ { "sample_id": "1", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "Img Background", "line": "unknown", "provenance": "Robust stock" }, "marker": "Actin", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "2", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "3", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "4", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "5", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "6", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "7", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "8", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "9", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "10", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "11", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "12", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "13", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "14", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "15", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "16", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "17", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "18", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "19", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "20", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "21", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": 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Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "125", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "126", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "127", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "128", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "129", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "130", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "131", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "132", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "133", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "134", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "135", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "136", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "137", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "138", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "139", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "140", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0024", "provenance": "Robust stock" }, "marker": "35S", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } } ] } }, "pedro": { "@xmlns:xsi": "http://www.w3.org/2001/XMLSchema-instance", "@xsi:schemaLocation": "/W:/communal/DataDescription/models/luc/model/newluc_model.xsd", "id": "will be updated by the system", "status": "in progress", "name": "210814H1", "purpose": "Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock", "description": "Plants entrained in 12L 12D white light.\n\nExperimental conditions (Red and Blue)\n12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, \nDawn is at ZT0 and dusk (shady dark) is at ZT12.", "comments": "Pedro is not able to record changes in intensity during an experiment, so please refer to the description & experimental conditions comment for true experimental conditions.\n\nPartner Experiment: 210814H2\n\nImages binned 2x2 to obtain signal from prr7prr9 CCR2:LUC line", "provenance": { "site": "Edinburgh", "author": "Yvette Wilson", "start_date": "2014-08-20" }, "growth_condition": { "name": "12L:12D", "medium": "Agar 1.2% MS 0.5", "incubation": "7 days at 4C", "growth_space": "Percival", "duration": "9", "comment": "Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.", "temperature_conditions": { "constant_temp": { "cycle_length": "24", "base_temp": "22" } }, "light_conditions": { "light_channel": { "spectrum": "white", "intensity": "90", "source": "tube", "diurnal_light": { "cycle_length": "24", "start_time": "0", "duration": "12" } } } }, "experimental_condition": { "name": "12L:12ShDx7", "medium": "Agar 1.2% MS 0.5", "growth_space": "Percival + LED", "duration": "7", "comment": "12 hours of bright light (90 umolm-2s-2) followed by 12 hours of 'shady dark' (6 umolm-2s-2) for 7 days\n\nThe first dawn starts at ZT0\n\nThe light intensities are controlled by the arduino.\n\nAt max light intensity red = 51 umolm-2s-1 and blue light intensity is 32 umol-2s-1.\n\nplants were transferred to experimental condiditons at ZT3", "temperature_conditions": { "constant_temp": { "cycle_length": "24", "base_temp": "22" } }, "light_conditions": { "light_channel": [ { "spectrum": "blue", "intensity": "32", "source": "LED", "diurnal_light": { "cycle_length": "24", "start_time": "0", "duration": "12" } }, { "spectrum": "red", "intensity": "51", "source": "LED", "diurnal_light": { "cycle_length": "24", "start_time": "0", "duration": "12" } } ] } }, "measurement": { "technique": "Luciferase luminescence", "equipment": "HAM 1", "parameters": "Imaging script started 1 hr 25 minutes after ZT0\n55mins wait, 5mins lights off, 30mins capture", "description": "Seedlings were treated on 9th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d10." }, "sample_preparation": { "method": "Plants sprayed with luciferin on day 9 of growth conditions and transferred to experimental conditions. Imaging was started on day 10." }, "samples": { "sample": [ { "sample_id": "1", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "Img Background", "provenance": "Robust stock" }, "marker": "Actin", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "2", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "3", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "4", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "5", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "6", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "7", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "8", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "9", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "10", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "11", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "12", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "13", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "14", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "15", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "16", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "17", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "18", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "19", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "prr7-3 prr9-1", "line": "G0011", "provenance": "Robust stock" }, "marker": "CCR2", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12ShDx7", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "20", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", 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