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"species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "G0062", "provenance": "Robust stock" }, "marker": "CAB", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "120", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "G0062", "provenance": "Robust stock" }, "marker": "CAB", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "121", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "G0062", "provenance": "Robust stock" }, "marker": "CAB", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "122", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "G0062", "provenance": "Robust stock" }, "marker": "CAB", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } } ] } }, "pedro": { "@xmlns:xsi": "http://www.w3.org/2001/XMLSchema-instance", "@xsi:schemaLocation": "/W:/communal/DataDescription/models/luc/model/newluc_model.xsd", "id": "will be updated by the system", "status": "in progress", "name": "010514H2", "purpose": "Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.", "description": "Experimental conditions (Red and Blue)\n12 hours light : 12 hours dark x4 followed by 3 days constant light.\nlight is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)", "comments": "Partner Experiment:010514H1", "provenance": { "site": "Edinburgh", "author": "Yvette Wilson", "start_date": "2014-04-23" }, "growth_condition": { "name": "12L:12D", "medium": "Agar 1.2% MS 0.5", "incubation": "7 days at 4C", "growth_space": "Percival", "duration": "6", "comment": "Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.", "temperature_conditions": { "constant_temp": { "cycle_length": "24", "base_temp": "22" } }, "light_conditions": { "light_channel": { "spectrum": "white", "intensity": "90", "source": "LED", "diurnal_light": { "cycle_length": "24", "start_time": "0", "duration": "12" } } } }, "experimental_condition": { "name": "12L:12D x4 LLx3", "medium": "Agar 1.2% MS 0.5", "growth_space": "Percival + LED", "duration": "7", "comment": "At max light intensity red = 51 umolm-2s-1 and blue light intensity is 32 umol-2s-1.\n\nplants were transferred to experimental condiditons at ZT3", "temperature_conditions": { "constant_temp": { "cycle_length": "24", "base_temp": "22" } }, "light_conditions": { "light_channel": [ { "spectrum": "red", "intensity": "51", "source": "LED", "light_complex_settings": { "light_days": [ { "start_day": "1", "duration": "4", "diurnal_light": { "cycle_length": "24", "start_time": "0", "duration": "12" } }, { "start_day": "5", "duration": "3", "constant_light": { "cycle_length": "24" } } ] } }, { "spectrum": "blue", "intensity": "32", "source": "LED", "light_complex_settings": { "light_days": [ { "start_day": "1", "duration": "4", "diurnal_light": { "cycle_length": "24", "start_time": "0", "duration": "12" } }, { "start_day": "5", "duration": "3", "constant_light": { "cycle_length": "24" } } ] } } ] } }, "measurement": { "technique": "Luciferase luminescence", "equipment": "HAM 2", "parameters": "Imaging script started 5 minutes before ZT0\n55mins wait, 5mins lights off, 30mins capture", "description": "Seedlings were treated on 6th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d7." }, "sample_preparation": { "method": "Plants sprayed with luciferin on day 6 of growth conditions and transferred to experimental conditions. Imaging was started on day 7." }, "samples": { "sample": [ { "sample_id": "1", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Img Background", "provenance": "Robust stock" }, "marker": "Actin", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "2", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "3", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "4", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "5", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "6", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "7", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "8", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "9", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": "Robust stock" }, "marker": "CCA1", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "10", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0007", "provenance": 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"sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "14", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "15", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "16", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "17", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "18", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "19", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "20", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "21", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "22", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0011", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "23", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "Wild Type", "line": "G0127", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "24", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "Wild Type", "line": "G0127", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "25", "sample_type": "seedling", "origin": "seedling", "growth_stage": 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"marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "28", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "Wild Type", "line": "G0127", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "29", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Columbia (Col)", "genotype": "Wild Type", "line": "G0127", "provenance": "Robust stock" }, "marker": "LHY", "growth_cond_name": "12L:12D", "experiment_cond_name": "12L:12D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "30", "sample_type": 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