| Property | Value |
|---|---|
| BioDare ID | 1130 |
| Author | Yvette Wilson |
| Institution | |
| License |
Plants entrained in 12L 12D white light.
Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Pedro is not able to record changes in intensity during an experiment, so please refer to the description & experimental conditions comment for true experimental conditions.
Partner Experiment: 240414H2
Plants sprayed with luciferin on day 6 of growth conditions and transferred to experimental conditions. Imaging was started on day 7.
Luciferase luminescence (HAM 1)
Seedlings were treated on 6th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d7.
Imaging script started 1 hr 25 minutes after ZT0 55mins wait, 5mins lights off, 30mins capture
Growth on Agar 1.2% MS 0.5 for 6.0 days (12L:12D). Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 12:00 | white | tube | 90 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12ShD x4 LL x3). 12 hours of bright light (90 umolm-2s-2) followed by 12 hours of 'shady dark' (6 umolm-2s-2) for four days and then 3 days of continuous light.
The first dawn starts at ZT0
The light intensities are controlled by the arduino.
At max light intensity red = 51 umolm-2s-1 and blue light intensity is 32 umol-2s-1.
plants were transferred to experimental condiditons at ZT3
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 0:00 | 12:00 | blue | LED | 32 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 32 |
| diurnal light | 4 | 24 | 0:00 | 12:00 | red | LED | 51 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 51 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |