| Property | Value |
|---|---|
| BioDare ID | 3188 |
| Author | Sarah Hodge |
| Institution | MPIMP - UEDIN |
| License |
ZT0 lights remain switched off until ZT3. At ZT3 light comes on at 90 umolm-2s-1. Dusk is lights off at ZT 12. Dark dawn cycle is for 4 days then continuous light (first day with dark dawn) for 3 days. ie 9L:15D x4 LLx3.
Control "dark" dawn experiment for double dawn experiment based on Virginie and Thiago's design (Stitt Lab).
Partner experiment: 140515H1
Plants sprayed with luciferin on day 6 of growth conditions, returned to entrainment conditions for a further 24hours, they were transferred to experimental conditions at ZT4 on d7. Imaging was started on day 8.
Luciferase luminescence (HAM 2)
Seedlings were treated on 6th day with Luciferin. They were then returned to entrainment condition for a further 24h (due to imaging set up problems). Seedlings were transferred to Experimental conditions at ZT4 on d7. Imaging was started on d8.
Imaging script started 5 minutes before ZT0 55mins wait, 5mins lights off, 30mins capture
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12D). Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment. The plants were entrained for 1 day more than standard conditions due problems with imaging set up.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 7 | 24 | 0:00 | 12:00 | white | tube | 90 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (3D:9L:12Dx3 LLx3). 4 days of 3 hours dark dawn, 9 hours of bright light 90 umolm-2-s-1, 12 hours dark followed by 3 days constant light, with dark dawn conditions on first dawn. The plates were transferred from entrainment conditions to experimental conditions at ZT4 on day 7.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 3:00 | 9:00 | red | LED | 55 |
| diurnal light | 1 | 24 | 3:00 | 21:00 | red | LED | 55 |
| constant light | 2 | 24 | 0:00 | 24:00 | red | LED | 55 |
| diurnal light | 4 | 24 | 3:00 | 9:00 | blue | LED | 35 |
| diurnal light | 1 | 24 | 3:00 | 21:00 | blue | LED | 35 |
| constant light | 2 | 24 | 0:00 | 24:00 | blue | LED | 35 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |