| Property | Value |
|---|---|
| BioDare ID | 1647 |
| Author | Yvette Wilson |
| Institution | |
| License |
12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Partner experiment: 030714H1
Double dawn experiment to compare to Mark Stitt's results
Plants sprayed with luciferin on day 6 of growth conditions and transferred to experimental conditions. Imaging was started on day 7.
Luciferase luminescence (HAM 2)
Seedlings were treated on 6th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d7.
Imaging script started 5 minutes before ZT0 55mins wait, 5mins lights off, 30mins capture
Growth on Agar 1.2% MS 0.5 for 6.0 days (12L:12D). Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 12:00 | white | tube | 90 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 3% Sucrose for 6.0 days (12L:12D +3% sucrose). Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 12:00 | white | tube | 90 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12Dx4 LLx3). The plates were exposed to dim light (lab lights) on the start of experimental day 1 and were transferred to experimental conditions at ZT3.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 0:00 | 12:00 | blue | tube | 32 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | tube | 32 |
| diurnal light | 4 | 24 | 0:00 | 12:00 | red | LED | 51 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 51 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5;Agar 1.2% MS 0.5 3% Sucrose for 7.0 days (12L12Dx4 LLx3 3%sucrose). The plates were exposed to dim light (lab lights) on the start of experimental day 1 and were transferred to experimental conditions at ZT3
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 24 | 0:00 | 12:00 | blue | LED | 32 |
| constant light | 3 | 24 | 0:00 | 24:00 | blue | LED | 32 |
| diurnal light | 4 | 24 | 0:00 | 12:00 | red | LED | 51 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 51 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |