| Property | Value |
|---|---|
| BioDare ID | 13637871113032 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Plants entrained in 14L 14D Experimental conditions: 14L14Dx4 LLx3
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Wrong imaging script was used (90mins) for T-28. 5mins, 10mins and 10mins of L was cut out during D1, D2 and D3 of imaging respectively.
Plants sprayed with luciferin on day 7 of growth conditions and transferred to imaging cabinet under experimental conditions.
Luciferase luminescence (HAM 1)
45mins wait, 5mins lights off, 25mins capture
Growth on Agar 1.2% MS 0.5 for 6.0 days (14L14D). Duration referred to T-cycle
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 28 | 0:00 | 14:00 | white | tube | 120 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 28 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (14L14D x 4 LL x 3). 5mins, 10mins and 10mins was cut out during D1, D2 and D3 of imaging due to the wrong script.
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 28 | 0:00 | 14:00 | blue | LED | 40 |
| constant light | 3 | 28 | 0:00 | 28:00 | blue | LED | 40 |
| diurnal light | 4 | 28 | 0:00 | 14:00 | red | LED | 72 |
| constant light | 3 | 28 | 0:00 | 28:00 | red | LED | 72 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 28 | 22 | -- | -- | -- |