| Property | Value |
|---|---|
| BioDare ID | 13643892291642 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Plants entrained in 7L 21D Experimental conditions: 7L21Dx4 LLx3
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
5mins of light at the end of daytime on imaging day 2 and day 3 appeared to be absent, using the 84mins imaging script. Hence, 6.5L:21.5D for those two days.
Plants sprayed with luciferin on day 7 of growth conditions and transferred to imaging cabinet under experimental conditions.
Luciferase luminescence (HAM 2)
54mins wait, 5mins lights off, 25mins capture
Growth on Agar 1.2% MS 0.5 for 6.0 days (7L 21D). Duration referred to T-cycle
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 28 | 0:00 | 7:00 | white | tube | 120 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 28 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (7L 21D). 5mins of light at the end of daytime on imaging day 2 and day 3 appeared to be absent, using the 84mins imaging script. Hence, 6.5L:21.5D for those two days
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 28 | 0:00 | 7:00 | blue | LED | 40 |
| constant light | 3 | 28 | 0:00 | 28:00 | blue | LED | 40 |
| diurnal light | 4 | 28 | 0:00 | 7:00 | red | LED | 72 |
| constant light | 3 | 28 | 0:00 | 28:00 | red | LED | 72 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 28 | 22 | -- | -- | -- |