| Property | Value |
|---|---|
| BioDare ID | 13649886952409 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Plants entrained in 15L 5D Experimental conditions: 15L 5Dx4 LLx3
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
5mins of light at the end of daytime during the first three imaging days appear to be absent, using the 75mins imaging script. Hence, 14.5L: 5.5D
Plants sprayed with luciferin on day 7 of growth conditions and transferred to imaging cabinet under experimental conditions.
Luciferase luminescence (HAM 2)
54mins wait, 5mins lights off, 25mins capture
Growth on Agar 1.2% MS 0.5 for 6.0 days (15L 5D). Duration referred to T-cycle
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 20 | 0:00 | 15:00 | white | tube | 120 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 20 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (15L 5Dx4 LLx3). 5mins of light at the end of daytime during the first three imaging days appear to be absent, using the 75mins imaging script. Hence, 14.5L 5.5D
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 4 | 20 | 0:00 | 15:00 | blue | LED | 40 |
| constant light | 3 | 20 | 0:00 | 20:00 | blue | LED | 40 |
| diurnal light | 4 | 20 | 0:00 | 15:00 | red | LED | 72 |
| constant light | 3 | 20 | 0:00 | 20:00 | red | LED | 72 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 20 | 22 | -- | -- | -- |