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"ELF4", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "166", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0013", "provenance": "Robust stock" }, "marker": "ELF4", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "ignored": "true", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "167", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0013", "provenance": "Robust stock" }, "marker": "ELF4", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "ignored": "false", "param": { "@name": "TAG", "@value": "Luc" } } ] } }, "pedro": { "@xmlns:xsi": "http://www.w3.org/2001/XMLSchema-instance", "@xsi:schemaLocation": "/C:/Honours Project/DataDescription/models/luc/model/newluc_model.xsd", "id": "130212H1", "status": "finished", "name": "130212H1: 5L15Dx4 LLx3 (T-20)", "purpose": "To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle", "description": "Plants entrained in 5L 15D \nExperimental conditions: 5L15Dx4 LLx3", "comments": "1.) 2H of L were cut short at the end of the first day of experimental condition (3L17D).\n2.) Fungal contamination of plate 1 (cca1/lhy TOC1:LUC, cca1/lhy/gi TOC1:LUC, WT TOC1:LUC) and plate 4 (WT CCA1:LUC, WT GI:LUC, WT ELF4:LUC). Seedlings selected for analysis were in fungal-free zone.\n3.) Plate 1 (cca1/lhy TOC1:LUC, cca1/lhy/gi TOC1:LUC, WT TOC1:LUC) was in different light intensity (room lightnings) for 30mins prior to experimental condition.", "provenance": { "site": "Edinburgh", "author": "Wei Ven Tee", "start_date": "2013-02-13" }, "growth_condition": { "name": "5L15D", "medium": "Agar 1.2% MS 0.5", "incubation": "6 days", "growth_space": "Percival", "duration": "6", "comment": "Duration referred to T-cycle", "temperature_conditions": { "constant_temp": { "cycle_length": "20", "base_temp": "22" } }, "light_conditions": { "light_channel": { "spectrum": "white", "intensity": "120", "source": "tube", "light_complex_settings": { "light_days": { "start_day": "1", "duration": "6", "diurnal_light": { "cycle_length": "20", "start_time": "0", "duration": "5" } } } } } }, "experimental_condition": { "name": "5L15D x4 LLx3", "medium": "Agar 1.2% MS 0.5", "growth_space": "Percival + LED", "duration": "7", "comment": "2H of L were cut short at the end of the first day of experimental condition (3L17D).", "temperature_conditions": { "constant_temp": { "cycle_length": "20", "base_temp": "22" } }, "light_conditions": { "light_channel": [ { "spectrum": "blue", "intensity": "40", "source": "LED", "light_complex_settings": { "light_days": [ { "start_day": "1", "duration": "4", "diurnal_light": { "cycle_length": "20", "start_time": "0", "duration": "5" } }, { "start_day": "5", "duration": "3", "constant_light": { "cycle_length": "20" } } ] } }, { "spectrum": "red", "intensity": "72", "source": "LED", "light_complex_settings": { "light_days": [ { "start_day": "1", "duration": "4", "diurnal_light": { "cycle_length": "20", "start_time": "0", "duration": "5" } }, { "start_day": "5", "duration": "3", "constant_light": { "cycle_length": "20" } } ] } } ] } }, "measurement": { "technique": "Luciferase luminescence", "equipment": "HAM 1", "parameters": "45mins wait, 5mins lights off, 25mins capture" }, "sample_preparation": { "method": "Plants sprayed with luciferin on day 7 of growth conditions and transferred to imaging cabinet under experimental conditions." }, "samples": { "sample": [ { "sample_id": "1", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Img Background", "provenance": "Robust stock" }, "marker": "Actin", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "2", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "cca1;lhy", "line": "M0023", "provenance": "Robust stock" }, "marker": "TOC1", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "3", "sample_type": "seedling", "origin": "seedling", 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"seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0013", "provenance": "Robust stock" }, "marker": "ELF4", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "166", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0013", "provenance": "Robust stock" }, "marker": "ELF4", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } }, { "sample_id": "167", "sample_type": "seedling", "origin": "seedling", "growth_stage": "seedling", "age": "7", "genetic_info": { "species": "Arabidopsis thaliana", "background": "Wasilewski (WS)", "genotype": "Wild Type", "line": "M0013", "provenance": "Robust stock" }, "marker": "ELF4", "growth_cond_name": "5L15D", "experiment_cond_name": "5L15D x4 LLx3", "param": { "@name": "TAG", "@value": "Luc" } } ] } }, "biblio": { "principal": "Andrew Millar", "institution": "University of Edinburgh", "author": [ "Wei Ven Tee", "Sarah Hodge" ] } }