| Property | Value |
|---|---|
| BioDare ID | 13618784212904 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Red)
Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Plants sprayed with luciferin on day 7 of growth conditions and transferred to imaging cabinet under experimental conditions
Luciferase luminescence (HAM 3)
Imagin script started 5 minutes before ZT 55mins wait, 5mins lights off, 30mins capture
Growth on Agar 1.2% MS 0.5 for 6.0 days (12L 12D). Plants transferred to imaging cabinet during 7th day
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| constant light | 6 | 24 | 0:00 | 24:00 | white | tube | 110 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (12R:12Dx3 RRx3). Plants sprayed with luciferin on 7th day of growth conditions and then transferred to imaging cabinet
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 3 | 24 | 0:00 | 12:00 | red | LED | 25 |
| constant light | 3 | 24 | 0:00 | 24:00 | red | LED | 25 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |