| Property | Value |
|---|---|
| BioDare ID | 13394437504756 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 20 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Testing spatiotemporal pattern of rhythmic gene expression to assay intercellular coupling, and comparing the strength of intercellular coupling in LL with entrainment in LD.
Start date is the date when imaging started. NB - this leaf had 3468 pixels, too many to handle in BioDare, so only 256 are declared here though the additional files attached have the data for all 3468.
Numerical data are provided as a 2D x time matrix in Matlab format (.mat), and as a text file with coordinates above each column of data. Please see the attached ReadMe file for notes on the data.
Twenty-one-day–old detached leaves were also placed between a slide and a coverslip lying on two layers of porous fiber tape and provided with water and nutriments through a thin piece of precision wipe tissue in contact with both the petiole and the liquid MS medium (Fig. 1A of PNAS paper).
Plants were sprayed with luciferin 48h before data collection started. Luciferin was also supplied in the liquid medium to which the leaf was transferred 24h before data collection started.
Luciferase luminescence (HAM 2)
As outlined in the PNAS paper.
15-minute exposures every 40 minutes. LEDs were switched off 5 mins before each image.
Growth on Agar 1.25% MS 0.5 for 21.0 days (LL growth).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| constant light | 21 | 24 | 0:00 | 24:00 | white | tube | 80 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 21 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (LL).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| constant light | 7 | 24 | 0:00 | 24:00 | red | LED | 20 |
| constant light | 7 | 24 | 0:00 | 24:00 | blue | LED | 20 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |