| Property | Value |
|---|---|
| BioDare ID | 13982027442235 |
| Author | Sarah Hodge |
| Institution | University of Edinburgh |
| License | CC_BY |
Plants entrained in 8L:16D (120umol m-2s-1) for 7 days. Experimental Conditions: 2 days 8L:16D, 4 days 16L:8D (red and blue light 60umolm-2s-1)
Re-entrainment dynamics during transition between light:dark cycles with different photoperiods, comparing Arabidopsis thaliana and Ostreococcus tauri. This expt in A. thaliana with transcriptional reporters.
PARTNER EXPERIMENT : 030810H3 LD-LD
sprayed on day 6 and returned to entrainment conditions. Plates transferred to experimental conditions at dawn on day 7.
Luciferase luminescence (HAM 3)
imaging script starts 5 minutes before required ZT, then 55 minutes interval, 5 minutes light off, 30 minutes capture
Growth on Agar 1.2% MS 0.5 for 7.0 days (8L:16D). Entrainment
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 7 | 24 | 0:00 | 8:00 | white | tube | 100 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 7.0 days (SD to LD). 2 days 8L:16D, 4 days 16L:8D. The light conditions are 60umolm-2s-1 (red and blue).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 2 | 24 | 0:00 | 8:00 | red | LED | 40 |
| diurnal light | 4 | 24 | 0:00 | 16:00 | red | LED | 40 |
| diurnal light | 2 | 24 | 0:00 | 8:00 | blue | LED | 20 |
| diurnal light | 4 | 24 | 0:00 | 16:00 | blue | LED | 20 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |