Edwards et al. (2010); WT in different light conditions.

Property Value
BioDare ID 13227622661196
Author Edwards, K.D.
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'Quantitative analysis of regulatory flexibility under changing environmental conditions.' by: Edwards, K.D..

    Theory suggested that multiple loops contribute the flexibility for molecular rhythms to track multiple phases of the external cycle. Clear dawn- and dusk-tracking rhythms illustrate the flexibility of timing in Ipomoea nil. Molecular clock components in Arabidopsis thaliana showed complex, photoperiod-dependent regulation.

Purpose

To investigate the dusk-sensing of clock genes and compare the results to mathematical models of the circadian clock.

Comments

Sample preparation

RNA was extracted and reverse transcribed as described previously (Locke et al, 2005b). Q-PCR was carried out using SYBR Green JumpStart Taq ReadyMix (Sigma, Gillingham, UK) in technical triplicate.

Measurement

Protocol

RT-PCR (Light Cycler 480 (Roche, UK))

Experimental conditions

growth_edwards_ph3

Growth on Agar 2% MS 1 3% Sucrose for 7.0 days (growth_edwards_ph3).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 7 24 0:00 3:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

growth_edwards_ph6

Growth on Agar 2% MS 1 3% Sucrose for 7.0 days (growth_edwards_ph6).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 7 24 0:00 6:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

growth_edwards_ph9

Growth on Agar 2% MS 1 3% Sucrose for 7.0 days (growth_edwards_ph9).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 7 24 0:00 9:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

growth_edwards_ph12

Growth on Agar 2% MS 1 3% Sucrose for 7.0 days (growth_edwards_ph12).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 7 24 0:00 12:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

growth_edwards_ph18

Growth on Agar 2% MS 1 3% Sucrose for 7.0 days (growth_edwards_ph18).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 7 24 0:00 18:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 7 24 22 -- -- --

experiment_edwards_ph3toLL

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph3toLL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 3:00 white LED 60
constant light 2 24 0:00 24:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

experiment_edwards_ph6toLL

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph6toLL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 6:00 white LED 60
constant light 2 24 0:00 24:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

experiment_edwards_ph9toLL

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph9toLL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 9:00 white LED 60
constant light 2 24 0:00 24:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

experiment_edwards_ph12toLL

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph12toLL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 12:00 white LED 60
constant light 2 24 0:00 24:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

experiment_edwards_ph18toLL

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph18toLL).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 18:00 white LED 60
constant light 2 24 0:00 24:00 white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

experiment_edwards_ph6toDD

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph6toDD).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 6:00 white LED 60
constant dark 2 24 -- -- white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

experiment_edwards_ph12toDD

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph12toDD).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 12:00 white LED 60
constant dark 2 24 -- -- white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

experiment_edwards_ph18toDD

Growth on Agar 2% MS 1 3% Sucrose for 3.0 days (experiment_edwards_ph18toDD).

Light

Type Duration (days) Cycle (h) Start Duration Spectrum Source Intensity
diurnal light 1 24 0:00 18:00 white LED 60
constant dark 2 24 -- -- white LED 60

Temperature

Type Duration (days) Cycle (h) Base (°C) Warm (°C) Warm start Warm duration
constant temperature 3 24 22 -- -- --

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