Effects of GA on clock in WT and prr9prr7
| Property |
Value |
| BioDare ID |
3838 |
| Author |
Daniel Seaton |
| Institution |
University of Edinburgh |
| License |
CC_BY |
Description
Purpose
To test whether GA has any effect on circadian clock period in the WT, and whether this effect is altered in the prr9prr7 mutant.
Comments
Sample preparation
Measurement
Protocol
Luciferase luminescence (HAM 2)
Experimental conditions
12L:12D
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12D).
Light
| Type |
Duration (days) |
Cycle (h) |
Start |
Duration |
Spectrum |
Source |
Intensity |
| diurnal light |
7 |
24 |
0:00 |
12:00 |
white |
tube |
100 |
Temperature
| Type |
Duration (days) |
Cycle (h) |
Base (°C) |
Warm (°C) |
Warm start |
Warm duration |
| constant temperature |
7 |
24 |
20 |
-- |
-- |
-- |
12L:12D to LL without GA
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12D to LL without GA).
Light
| Type |
Duration (days) |
Cycle (h) |
Start |
Duration |
Spectrum |
Source |
Intensity |
| diurnal light |
2 |
24 |
0:00 |
12:00 |
white |
LED |
80 |
| constant light |
5 |
24 |
0:00 |
24:00 |
white |
LED |
80 |
Temperature
| Type |
Duration (days) |
Cycle (h) |
Base (°C) |
Warm (°C) |
Warm start |
Warm duration |
| constant temperature |
7 |
24 |
22 |
-- |
-- |
-- |
12L:12D to LL with GA
Growth on Agar 1.2% MS 0.5 for 7.0 days (12L:12D to LL with GA).
Light
| Type |
Duration (days) |
Cycle (h) |
Start |
Duration |
Spectrum |
Source |
Intensity |
| diurnal light |
2 |
24 |
0:00 |
12:00 |
white |
LED |
80 |
| constant light |
5 |
24 |
0:00 |
24:00 |
white |
LED |
80 |
Temperature
| Type |
Duration (days) |
Cycle (h) |
Base (°C) |
Warm (°C) |
Warm start |
Warm duration |
| constant temperature |
7 |
24 |
22 |
-- |
-- |
-- |
Files
- metamorph_output_abridged.2.xlsx
- 300915_Ham2_GA_p97_experiment.csv