| Property | Value |
|---|---|
| BioDare ID | 3492 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Literature data from: 'Temp' by: Aurora Pinas-Fernandez.
Wild-type and clock-mutant seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees, every 2h, for 24h.
Experiment to reparameterize the clock model without exogenous sucrose
24h sample lost for several genotypes. Curated by Andrew Millar. AJM manually edited the 14h to 18h timepoints in the prr7-9- mutant, as many values were omitted when the ratio's looked reasonably close among reps. For toc1-9, ignore LHY data, much lower than CCA1 in first 4 timepoints. These datapoints removed from file.
Samples were harvested into Ambion RNAlater for some weeks, at 4C and -20C. RNA extraction was done following protocol of GE Health Care-illustra RNAspin 96RNA Isolation kit. Samples were spec'd with Nanodrop. cDNA synthesis was done with Super Script Vilo kit (Invitrogene) from 1ug RNA.
qRT-PCR (Robot + Light Cycler 480)
Literature data from: '' by: .
primers unknown
uknown
Growth on Agar 1.2% MS 0.5 for 7.0 days (Entrainment 22C Diurnal).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 7 | 24 | 0:00 | 12:00 | white | tube | 50 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 7 | 24 | 22 | -- | -- | -- |
Growth on Agar 1.2% MS 0.5 for 6.0 days (17C LD (12:12) Off Sucrose).
| Type | Duration (days) | Cycle (h) | Start | Duration | Spectrum | Source | Intensity |
|---|---|---|---|---|---|---|---|
| diurnal light | 6 | 24 | 0:00 | 12:00 | white | tube | 50 |
| Type | Duration (days) | Cycle (h) | Base (°C) | Warm (°C) | Warm start | Warm duration |
|---|---|---|---|---|---|---|
| constant temperature | 6 | 24 | 17 | -- | -- | -- |