#Item name	Detection of lavandulol in extracts of engineered fungi
Lab manager	Jaime Primo
Sample collection protocol	SxF lines provided by Elena Moreno
Extraction protocol for liquid cultures	Liquid-liquid extraction with 50mL ethyl acetate:hexane (7:3) (x3 times). Concentration up to 1mL and syringe filtration.
Extraction protocol for mycelia	Solvent extraction with 50 mL ethyl acetate:hexane (7:3) and magnetic agitation for 2 hours. Concentration up to 1mL and syringe filtration.
Chromatography protocol	1ul injection, oven temperature program: 60C for 1 min, raise at 10C/min up to 120C, mantained for 4 min; then, raise at 10C/min up to 130C, and finally raised at 20C/min up to 280C, hold for 2 min.
Mass spectrometry protocol	FS mode:39 to 450 uma at 70 eV. SRM mode: lavandulol - 69-41 at 5eV, 93-77 at 10eV