#Item name	quantification of pheromone emitted by non-truncated SxP plants by GC/MS-MS in SRM mode
Creation date	20-Nov-21
#Extraction
Extract ID	PhEm
Extract Name	PhEm_non-truncated
Extraction Method	Dynamic volatile collection using 400 mg Porapak Q and further elution of volatiles with 5 mL pentane
Date Extraction	
#Post Extraction
Derivatization or Labelling	none	
Date Derivatization or Labelling	none
Derivatized or labeled Extract ID	none
Derivatized or labeled Extract Name	none
Other Post Extraction Procedures	concentration under nitrogen stream to 0.2 mL
Storage	direct analysis
#Chromatography
Date GC-MS Run	
GC Instrument	Trace 1300 Thermo Scientific
GC Autosampler Model 	AI 1310 Thermo Scientific	
GC Column model	Zebron ZB5-MS
GC Column type	fused silica capillary column 30mx0.25x0.25
Guard Column	none
#Mass spectrometry
MS Scan polarity	positive
MS Scan mz range	transitions for pheromone compounds 82-67 5eV, 95-67 10eV, 96-54 10 eV and 96-81 5eV
MS Instrument	TSQ 8000 Evo Thermo Scientific
MS Ion source	electron ionization (EI)
Mass analyzer	triple quadrupole mass filter
Raw Spectral Data Files	Blank
#Other
Operator	Sandra Vacas
Notes