#Item name	quantification of pheromone content in fresh leaves of SxP plants by GC/MS-MS in SRM mode
Creation date	20-Nov-21
#Extraction
Extract ID	PhCont
Extract Name	PhCont_fresh
Extraction Method	Solvent extraction with 10 mL toluene (per 3g leaves) with magnetic agitation for 12h. Plant material previously grounded with sand.
Date Extraction	
#Post Extraction
Derivatization or Labelling	none	
Date Derivatization or Labelling	none
Derivatized or labeled Extract ID	none
Derivatized or labeled Extract Name	none
Other Post Extraction Procedures	filtration of 1 mL of the extract
Storage	direct analysis
#Chromatography
Date GC-MS Run	
GC Instrument	Trace 1300 Thermo Scientific
GC Autosampler Model 	AI 1310 Thermo Scientific	
GC Column model	Zebron ZB5-MS
GC Column type	fused silica capillary column 30mx0.25x0.25
Guard Column	none
#Mass spectrometry
MS Scan polarity	positive
MS Scan mz range	transitions for pheromone compounds 82-67 5eV, 95-67 10eV, 96-54 10 eV and 96-81 5eV
MS Instrument	TSQ 8000 Evo Thermo Scientific
MS Ion source	electron ionization (EI)
Mass analyzer	triple quadrupole mass filter
Raw Spectral Data Files	Blank
#Other
Operator	Sandra Vacas
Notes