#Item name	GC-MS-EAD runs to test biological activity of plant produced pheromone
Creation date	20-Nov-21
#Extraction
Extract ID	SxPv1-2
Extract Name	SxPv1-2 fractions
Extraction Method	Solvent extraction with 40 mL toluene (per 10g leaves) with magnetic agitation for 12h. Plant material previously grounded with sand.
Date Extraction	
#Post Extraction
Derivatization or Labelling	none	
Date Derivatization or Labelling	none
Derivatized or labeled Extract ID	none
Derivatized or labeled Extract Name	none
Other Post Extraction Procedures	fractionation of the extract in a gravity column using silical gel as stationary phase and hexane-eter as solvent. Selection of fractions containing biosynthetic Z11C16OL for EAG.
Storage	direct analysis
#Chromatography
Date GC-MS Run	
GC Instrument	Clarus 600 GC/MS
GC Autosampler Model 	Clarus 600 GC/MS	
GC Column model	Zebron ZB5-MS
GC Column type	fused silica capillary column 30mx0.25x0.25
Guard Column	none
#Mass spectrometry
MS Scan polarity	positive
MS Scan mz range	40-500
MS Instrument	Clarus 600 MS
MS Ion source	electron ionization (EI)
Mass analyzer	simple quadrupole
Raw Spectral Data Files	Blank
#Electroantennography
Instrument	Syntech CS-55 stimulus controller
Air flow	50 mlmin
Stimuli deliverer	GC stream
#Other
Operator	Sandra Vacas
Notes