#Item name	Pheromone detection in fungi mycelia
Lab manager	Jaime Primo
Sample collection protocol	SxF fungi provided by Elena Moreno
Extraction protocol	Solvent extraction of mycelia with 50mL ethyl acetate for 1h with magentic stirring. Concentration up to 1mL and syringe filtration.
Chromatography protocol	1ul injection, oven temperature program: 60C for 1 min, raise at 10C/min up to 110C, mantained for 5 min; then, raise at 10C/min up to 150C, mantained for 3 min, and finally raised at 10C/min up to 300C, hold for 1 min.
Mass spectrometry protocol	FS mode:39 to 450 uma at 70 eV. SRM mode:82-67 at 5eV, 95-67 at 10eV, 96-54 at 10eV, and 96-81 at 5eV.