#Item name	Pheromone detection in fungi liquid culture
Lab manager	Jaime Primo
Sample collection protocol	SxF fungi provided by Elena Moreno
Extraction protocol	Liquid-liquid extraction with 30mL ethyl acetate (x3 times). Concentration up to 1mL and syringe filtration.
Chromatography protocol	1ul injection, oven temperature program: 60C for 1 min, raise at 10C/min up to 110C, mantained for 5 min; then, raise at 10C/min up to 150C, mantained for 3 min, and finally raised at 10C/min up to 300C, hold for 1 min.
Mass spectrometry protocol	FS mode:39 to 450 uma at 70 eV. SRM mode:82-67 at 5eV, 95-67 at 10eV, 96-54 at 10eV, and 96-81 at 5eV.