In this assay, we performed a stable transformation in Nicotiana benthamiana plants, over a dCasEV genetic background.
1 plant over the 11 regenerated and tested as PCR positive plants was a producer plant, and their sex pheromone production levels are here reported.
In this study, as mentioned in the others short reports, the positive plants were also producing other specific volatiles when analyzed by GCMS, like hexadecanol and hexadecanyl acetate, which were not detected in the analyzed plants of SxP v1 (v1.0 or v1.2). This results should be further analyzed in the future, in order to determine if this secondary production is due to the different configuration of the genes compared to SxPv1..