Assay:	_A_20211221-LUM
Short Name:	20211221-LUM
Assay Class:	WET
Assay Type:	LUM
Title:	dCasEV synthetic promoters with NanoLuc reporter system
Description:	Test for dCasEV synthetic promoters using EI reporter system based on Nanoluciferase as reporter and luciferase as reference signal.
pISA Assay creation date:	2021-12-22
pISA Assay creator:	ElenaMG
Lab manager:	DO
Operator:	ElenaMG
Link to SynBio DM:	https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703
Link to protocols:	../protocols/LUCIFERASE
Additional Notes:	
Phenodata:	../../phenodata_Luciferase_20211222.txt
Featuredata:	
Plate ID:	08
DNA introduction system:	agrobacteria mediated transient transformation
SynBio GenotypeID(s):	*
Date of transformation/transfection:	2021-12-15
Treatment:	Copper
Date of treatment:	2021-12-18
Date of sampling:	2021-12-20
Date of luminescence assay start:	2021-12-21
Time of luminescence assay start:	18:00
Luminometer:	Promega GloMax 20 20
Protocol (luminometer setup):	Luminescence 3x read
Time [min] between two measurements (luminometer setup):	2
Integration time [ms] (luminometer setup):	10000
Temperature (luminometer) at start of the experiment [degrees Celsius]:	24
Temperature (luminometer) at the end of the experiment [degrees Celsius]:	24
Time between start of sampling and start of experiment [min]:	60
Silencing supressor added:	p19
Notes:	
Data: