Assay:	_A_20201112-LUM
Short Name:	20201112-LUM
Assay Class:	WET
Assay Type:	LUM
Title:	Luciferase assay re-testing US6.X
Description:	The US6.X series was tested again using separated plasmids for the gRNAs and the dCas9EV system (so far it was used a plasmid containig the system and the guide together)
pISA Assay creation date:	2020-11-12
pISA Assay creator:	ElenaMG
Lab manager:	DO
Operator:	ElenaMG
Link to SynBio DM:	https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703
Link to protocols:	../protocols/LUCIFERASE
Additional Notes:	
Phenodata:	../../phenodata_Luciferase_20211222.txt
Featuredata:	
Plate ID:	07
DNA introduction system:	agrobacteria mediated transient transformation
Date of transformation/transfection:	2020-11-07
Treatment:	none
Date of treatment:	2020-11-07
Date of sampling:	2020-11-12
Date of luminescence assay start:	2020-11-12
Time of luminescence assay start:	12:00
Luminometer:	GloMax Multidetection system (Promega)
Protocol (luminometer setup):	Luminiscence 3x read
Time [min] between two measurements (luminometer setup):	2
Integration time [ms] (luminometer setup):	10000
Temperature (luminometer) at start of the experiment [degrees Celsius]:	24
Temperature (luminometer) at the end of the experiment [degrees Celsius]:	24
Time between start of sampling and start of experiment [min]:	60
Silencing supressor added:	p19
Notes:	
Data: