Assay: _A_20200818-LUM Short Name: 20200818-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay of new minimal promoters Description: New minimal promoters were designed and tested to increase the battery of pieces available for creating synthetic promoters pISA Assay creation date: 2020-08-18 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703 Link to protocols: ../protocols/LUCIFERASE Additional Notes: Phenodata: ../../phenodata_Luciferase_20211222.txt Featuredata: Plate ID: 06 DNA introduction system: agrobacteria mediated transient transformation Date of transformation/transfection: 2020-08-13 Treatment: none Date of treatment: 2020-08-13 Date of sampling: 2020-08-18 Date of luminescence assay start: 2020-08-19 Time of luminescence assay start: 12:00 Luminometer: GloMax Multidetection system (Promega) Protocol (luminometer setup): Luminiscence 3x read Time [min] between two measurements (luminometer setup): 2 Integration time [ms] (luminometer setup): 10000 Temperature (luminometer) at start of the experiment [degrees Celsius]: 24 Temperature (luminometer) at the end of the experiment [degrees Celsius]: 24 Time between start of sampling and start of experiment [min]: 60 Silencing supressor added: p19 Notes: Data: